Fast volumetric imaging with patterned illumination via digital micro-mirror devicebased temporal focusing multiphoton microscopy

Chia Yuan Chang, Yvonne Yuling Hu, Chun Yu Lin, Cheng Han Lin, Hsin Yu Chang, Sheng Feng Tsai, Tzu Wei Lin, Shean Jen Chen

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Temporal focusing multiphoton microscopy (TFMPM) has the advantage of area excitation in an axial confinement of only a few microns; hence, it can offer fast three-dimensional (3D) multiphoton imaging. Herein, fast volumetric imaging via a developed digital micromirror device (DMD)-based TFMPM has been realized through the synchronization of an electron multiplying charge-coupled device (EMCCD) with a dynamic piezoelectric stage for axial scanning. The volumetric imaging rate can achieve 30 volumes per second according to the EMCCD frame rate of more than 400 frames per second, which allows for the 3D Brownian motion of one-micron fluorescent beads to be spatially observed. Furthermore, it is demonstrated that the dynamic HiLo structural multiphoton microscope can reject background noise by way of the fast volumetric imaging with high-speed DMD patterned illumination.

原文English
頁(從 - 到)1727-1736
頁數10
期刊Biomedical Optics Express
7
發行號5
DOIs
出版狀態Published - 2016 五月 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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