Fibronectin in cell adhesion and migration via N-glycosylation

Cheng Te Hsiao, Hung Wei Cheng, Chi Ming Huang, Hao Ru Li, Meng Hsin Ou, Jie Rong Huang, Kay Hooi Khoo, Helen Wenshin Yu, Yin Quan Chen, Yang Kao Wang, Arthur Chiou, Jean Cheng Kuo

研究成果: Article

10 引文 (Scopus)

摘要

Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.

原文English
頁(從 - 到)70653-70668
頁數16
期刊Oncotarget
8
發行號41
DOIs
出版狀態Published - 2017 一月 1

指紋

Glycosylation
Fibronectins
Cell Adhesion
Cell Movement
Swine
Wound Healing
Extracellular Matrix
Polysaccharides
Bandages
Integrins
Glycoproteins
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Oncology

引用此文

Hsiao, C. T., Cheng, H. W., Huang, C. M., Li, H. R., Ou, M. H., Huang, J. R., ... Kuo, J. C. (2017). Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget, 8(41), 70653-70668. https://doi.org/10.18632/oncotarget.19969
Hsiao, Cheng Te ; Cheng, Hung Wei ; Huang, Chi Ming ; Li, Hao Ru ; Ou, Meng Hsin ; Huang, Jie Rong ; Khoo, Kay Hooi ; Yu, Helen Wenshin ; Chen, Yin Quan ; Wang, Yang Kao ; Chiou, Arthur ; Kuo, Jean Cheng. / Fibronectin in cell adhesion and migration via N-glycosylation. 於: Oncotarget. 2017 ; 卷 8, 編號 41. 頁 70653-70668.
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Hsiao, CT, Cheng, HW, Huang, CM, Li, HR, Ou, MH, Huang, JR, Khoo, KH, Yu, HW, Chen, YQ, Wang, YK, Chiou, A & Kuo, JC 2017, 'Fibronectin in cell adhesion and migration via N-glycosylation', Oncotarget, 卷 8, 編號 41, 頁 70653-70668. https://doi.org/10.18632/oncotarget.19969

Fibronectin in cell adhesion and migration via N-glycosylation. / Hsiao, Cheng Te; Cheng, Hung Wei; Huang, Chi Ming; Li, Hao Ru; Ou, Meng Hsin; Huang, Jie Rong; Khoo, Kay Hooi; Yu, Helen Wenshin; Chen, Yin Quan; Wang, Yang Kao; Chiou, Arthur; Kuo, Jean Cheng.

於: Oncotarget, 卷 8, 編號 41, 01.01.2017, p. 70653-70668.

研究成果: Article

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AU - Khoo, Kay Hooi

AU - Yu, Helen Wenshin

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AU - Wang, Yang Kao

AU - Chiou, Arthur

AU - Kuo, Jean Cheng

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N2 - Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.

AB - Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.

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Hsiao CT, Cheng HW, Huang CM, Li HR, Ou MH, Huang JR 等. Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget. 2017 1月 1;8(41):70653-70668. https://doi.org/10.18632/oncotarget.19969