Flow cytometric determination for dengue virus-infected cells: Its application for antibody-dependent enhancement study

Kao Jean Huang, Yu Ching Yang, Yee Shin Lin, Hsiao Sheng Liu, Trai Ming Yeh, Shun Hua Chen, Ching Chuan Liu, Huan Yao Lei

研究成果: Article

8 引文 斯高帕斯(Scopus)

摘要

The theory of antibody-dependent enhancement plays an important role in the dengue virus infection. However, its molecular mechanism is not clearly studied partially due to lack of a sensitive assay to determine the dengue virus-infected cells. We developed a flow cytometric assay with anti-dengue antibody intracellular staining on dengue virus-infected cells. Both anti-E and anti-prM Abs could enhance the dengue virus infection. The anti-prM Ab not only enhanced the dengue virus infected cell mass, but also increased the dengue virus protein synthesis within the cells. The effect of anti-prM Ab-mediated enhancement on dengue virus infection is serotype-independent. We concluded that the target cell-based flow cytometry with anti-dengue antibody intracellular staining on dengue virus-infected cells is a sensitive assay to detect the dengue virus infected cells and to evaluate the effect of enhancing antibody on dengue virus infection on cell lines or human primary monocytes.

原文English
頁(從 - 到)142-150
頁數9
期刊Dengue Bulletin
29
出版狀態Published - 2005 十二月 1

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

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