Fluorescence Signal-to-Noise Ratio Enhanced by Off-Plane Excitation for Quantitative PCR Device

Liang Chieh Chao, Hsin Yi Tsai, Cheng Ru Li, Kuo Cheng Huang, Yu Hsuan Lin, Dar Bin Shieh

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)

摘要

The fluorescence signal of Sybr Green can represent the amount of target DNA copy numbers in the samples, thereby determine a positive or negative diagnostic results after polymerase chain reaction (PCR). For precision quantification of the target gene in the sample, the fluorescent signal should be recorded in each cycle. Traditionally, highly sensitive devices such as silicon photomultipliers and high-power light source such as laser diode were used to excite and detect the fluorescence from double strand DNA selective dyes. However, the costs of these components were too high to be widely applied for rapidly on-site screening of outbreak causing pathgens in local clinics and fields of tests. In this study, low-cost optical element such as LED and photodiode were used to implementan off-plane excitation method to enhance the fluorescence signal. Several parameters such as illumination angle between LED and photodiode in horizontal direction and tilted illumination in vertical direction were optimized in the experiment to ensure the performance. The results showed that the fluorescence intensity can be enhanced approximately 25 %. These results indicated that the developed illumination architecture had advantages of low background noise, high sensitivity and high fluorescence variation. This research has successfully demonstrated an off-plane excitation method for enhancing the resolution of qPCR system and applying in medical diagnostics, rapid screening and related applications.

原文English
主出版物標題IEEE Medical Measurements and Applications, MeMeA 2020 - Conference Proceedings
發行者Institute of Electrical and Electronics Engineers Inc.
ISBN(電子)9781728153865
DOIs
出版狀態Published - 2020 6月
事件15th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2020 - Bari, Italy
持續時間: 2020 6月 12020 6月 3

出版系列

名字IEEE Medical Measurements and Applications, MeMeA 2020 - Conference Proceedings

Conference

Conference15th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2020
國家/地區Italy
城市Bari
期間20-06-0120-06-03

All Science Journal Classification (ASJC) codes

  • 生物醫學工程
  • 醫藥(雜項)
  • 健康資訊學
  • 復健
  • 儀器

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