Fluorescent immunosensing enhanced with a bead-based optoelectrokinetic platform

Han Sheng Chuang, Hsiao Neng Lin, Jen Yi Wang

研究成果: Conference contribution

摘要

Bead-based immunosensing is a promising technology in the point-of-care diagnostics due to its high flexibility. For dilute samples, functionalized particles can concentrate dispersed analytes and act as carriers for particle manipulation. To realize rapid and selective diagnosis, a bead-based optoelectrokinetic immunosensing technique, termed rapid electrokinetic patterning (REP), was employed herein to detect a biomarker, lipocalin 1 (LCN1), for diabetic retinopathy. The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The detection required only several tens of seconds. The limit of detection (LOD) reached nearly 110 pg/mL. The combined use of bead-based immunoassays and the optoelectrokinetic platform enabled a noninvasive measure to the early diagnosis of diabetic retinopathy.

原文English
主出版物標題Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019
發行者Institute of Electrical and Electronics Engineers Inc.
頁面130-134
頁數5
ISBN(電子)9781728116297
DOIs
出版狀態Published - 2019 4月
事件14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019 - Bangkok, Thailand
持續時間: 2019 4月 112019 4月 14

出版系列

名字Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019

Conference

Conference14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019
國家/地區Thailand
城市Bangkok
期間19-04-1119-04-14

All Science Journal Classification (ASJC) codes

  • 機械工業
  • 電子、光磁材料
  • 表面、塗料和薄膜
  • 儀器

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