TY - JOUR
T1 - Formation and characterization of extracellular polymeric substance from Shewanella xiamenensis BC01 under calcium stimulation
AU - Chen, Tingting
AU - Zhou, Yunli
AU - Ng, I. Son
AU - Yang, Chun Shan
AU - Wang, Hsiang Yu
N1 - Funding Information:
The authors are grateful to the startup fund from the National Cheng Kung University and the Ministry of Science and Technology in Taiwan under Grant MOST 103-2218-E-006-027-MY2 .
Publisher Copyright:
© 2015 Taiwan Institute of Chemical Engineers.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The widely distributed genus Shewanella is famous for its capacity of electron transferring and extracellular polymeric substance (EPS) production. Regarding to the EPS production by Shewanella is a critical event for its application, the aim of this study is to decipher the effect of calcium ion on a new S. xiamenensis BC01 (SXM). It grew and survived at a high concentration of 20 mM CaCl2 in LB medium. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that calcium had an effect on cell resulted in the stimulating EPS production and changing morphology of nano-filament. Further characterization of the EPS through attenuated total reflectance of Fourier transform infrared (ATR-FTIR) spectroscopy, and protein identification of whole cell via SDS-PAGE coupled with tandem mass spectrometry implied calcium affected membrane and flagellum structure. The flagellin domain-containing protein was found to be relative with the process of flagella assembling, which enhanced EPS formation. Finally, the production of EPS increased from 0.35 g/L to 1.7 4g/L was corresponding to the increment of calcium. Thus, the decolorization rate of azo dye reactive blue by EPS was increased from 26.5 % to 49.5 % upon the addition of calcium.
AB - The widely distributed genus Shewanella is famous for its capacity of electron transferring and extracellular polymeric substance (EPS) production. Regarding to the EPS production by Shewanella is a critical event for its application, the aim of this study is to decipher the effect of calcium ion on a new S. xiamenensis BC01 (SXM). It grew and survived at a high concentration of 20 mM CaCl2 in LB medium. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that calcium had an effect on cell resulted in the stimulating EPS production and changing morphology of nano-filament. Further characterization of the EPS through attenuated total reflectance of Fourier transform infrared (ATR-FTIR) spectroscopy, and protein identification of whole cell via SDS-PAGE coupled with tandem mass spectrometry implied calcium affected membrane and flagellum structure. The flagellin domain-containing protein was found to be relative with the process of flagella assembling, which enhanced EPS formation. Finally, the production of EPS increased from 0.35 g/L to 1.7 4g/L was corresponding to the increment of calcium. Thus, the decolorization rate of azo dye reactive blue by EPS was increased from 26.5 % to 49.5 % upon the addition of calcium.
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U2 - 10.1016/j.jtice.2015.05.031
DO - 10.1016/j.jtice.2015.05.031
M3 - Article
AN - SCOPUS:84930697572
SN - 1876-1070
VL - 57
SP - 175
EP - 181
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
ER -