The target of this research is to design and fabricate the micro-heater and its electrodes in order to study the thermal bubble behavior. In fabrication of micro-heater, this research focus on the design of multilayer micro-heater and the selection of materials further. For the multilayer materials, we choose Ti/Al/Ta (15/25/200 nm/nm/nm) to be the material to replace the conventional polysilicon, Ta or expensive Pt heaters for improving the heat-resistant ability of Pt, poor adhesion of Ta and high electrical resistance of polysilicon. Then, the bubble sizes between frequencies and voltages were discussed in the multilayer heating materials. The multilayer micro-heater in this research can generate good bubbles at low frequencies operation. It has the advantages of low electrical resistance, the good thermal resistance, and the good heat dissipation to contribute to improve the poor heat dissipation and strength of the Pt microheater in order to increase the life of the heater.