From midbody protein - Protein interaction network construction to novel regulators in cytokinesis

Tzu Chi Chen, Sheng An Lee, Tse-Ming Hong, Jin Yuan Shih, Jin Mei Lai, Hsin Ying Chiou, Shuenn Chen Yang, Chen Hsiung Chan, Cheng Yan Kao, Pan Chyr Yang, Chi Ying F. Huang

研究成果: Article

19 引文 (Scopus)

摘要

Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in cytokinesis, the z-score, a standard statistic score, rather than hub degree was implemented to prioritize the novel hubs. Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. Moreover, ectopic expression of CRMP-1 mutant in which Aurora-A phosphorylation sites have been replaced with Ala results in a cytokinesis defect. This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis.

原文English
頁(從 - 到)4943-4953
頁數11
期刊Journal of Proteome Research
8
發行號11
DOIs
出版狀態Published - 2009 十一月 6

指紋

Protein Interaction Maps
Cytokinesis
Proteome
Defects
Phosphorylation
Proteins
Statistics
Organelles
Fluorescent Antibody Technique
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

引用此文

Chen, T. C., Lee, S. A., Hong, T-M., Shih, J. Y., Lai, J. M., Chiou, H. Y., ... Huang, C. Y. F. (2009). From midbody protein - Protein interaction network construction to novel regulators in cytokinesis. Journal of Proteome Research, 8(11), 4943-4953. https://doi.org/10.1021/pr900325f
Chen, Tzu Chi ; Lee, Sheng An ; Hong, Tse-Ming ; Shih, Jin Yuan ; Lai, Jin Mei ; Chiou, Hsin Ying ; Yang, Shuenn Chen ; Chan, Chen Hsiung ; Kao, Cheng Yan ; Yang, Pan Chyr ; Huang, Chi Ying F. / From midbody protein - Protein interaction network construction to novel regulators in cytokinesis. 於: Journal of Proteome Research. 2009 ; 卷 8, 編號 11. 頁 4943-4953.
@article{660cbf1f8d6041faa219c5d03cbfe40b,
title = "From midbody protein - Protein interaction network construction to novel regulators in cytokinesis",
abstract = "Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in cytokinesis, the z-score, a standard statistic score, rather than hub degree was implemented to prioritize the novel hubs. Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. Moreover, ectopic expression of CRMP-1 mutant in which Aurora-A phosphorylation sites have been replaced with Ala results in a cytokinesis defect. This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis.",
author = "Chen, {Tzu Chi} and Lee, {Sheng An} and Tse-Ming Hong and Shih, {Jin Yuan} and Lai, {Jin Mei} and Chiou, {Hsin Ying} and Yang, {Shuenn Chen} and Chan, {Chen Hsiung} and Kao, {Cheng Yan} and Yang, {Pan Chyr} and Huang, {Chi Ying F.}",
year = "2009",
month = "11",
day = "6",
doi = "10.1021/pr900325f",
language = "English",
volume = "8",
pages = "4943--4953",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "11",

}

Chen, TC, Lee, SA, Hong, T-M, Shih, JY, Lai, JM, Chiou, HY, Yang, SC, Chan, CH, Kao, CY, Yang, PC & Huang, CYF 2009, 'From midbody protein - Protein interaction network construction to novel regulators in cytokinesis', Journal of Proteome Research, 卷 8, 編號 11, 頁 4943-4953. https://doi.org/10.1021/pr900325f

From midbody protein - Protein interaction network construction to novel regulators in cytokinesis. / Chen, Tzu Chi; Lee, Sheng An; Hong, Tse-Ming; Shih, Jin Yuan; Lai, Jin Mei; Chiou, Hsin Ying; Yang, Shuenn Chen; Chan, Chen Hsiung; Kao, Cheng Yan; Yang, Pan Chyr; Huang, Chi Ying F.

於: Journal of Proteome Research, 卷 8, 編號 11, 06.11.2009, p. 4943-4953.

研究成果: Article

TY - JOUR

T1 - From midbody protein - Protein interaction network construction to novel regulators in cytokinesis

AU - Chen, Tzu Chi

AU - Lee, Sheng An

AU - Hong, Tse-Ming

AU - Shih, Jin Yuan

AU - Lai, Jin Mei

AU - Chiou, Hsin Ying

AU - Yang, Shuenn Chen

AU - Chan, Chen Hsiung

AU - Kao, Cheng Yan

AU - Yang, Pan Chyr

AU - Huang, Chi Ying F.

PY - 2009/11/6

Y1 - 2009/11/6

N2 - Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in cytokinesis, the z-score, a standard statistic score, rather than hub degree was implemented to prioritize the novel hubs. Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. Moreover, ectopic expression of CRMP-1 mutant in which Aurora-A phosphorylation sites have been replaced with Ala results in a cytokinesis defect. This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis.

AB - Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in cytokinesis, the z-score, a standard statistic score, rather than hub degree was implemented to prioritize the novel hubs. Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. Moreover, ectopic expression of CRMP-1 mutant in which Aurora-A phosphorylation sites have been replaced with Ala results in a cytokinesis defect. This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis.

UR - http://www.scopus.com/inward/record.url?scp=70449347343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70449347343&partnerID=8YFLogxK

U2 - 10.1021/pr900325f

DO - 10.1021/pr900325f

M3 - Article

C2 - 19799413

AN - SCOPUS:70449347343

VL - 8

SP - 4943

EP - 4953

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 11

ER -