Functional analysis of an orange-spotted grouper (Epinephelus coioides) interferon gene and characterisation of its expression in response to nodavirus infection

Young Mao Chen, Cham En Kuo, Guan Ru Chen, Yu Ting Kao, Jun Zou, Chris J. Secombes, Tzong-Yueh Chen

研究成果: Article

29 引文 斯高帕斯(Scopus)

摘要

We cloned and sequenced 2C I-IFN, a two-cysteine containing type I interferon (I-IFN) gene, in orange-spotted grouper (Epinephelus coioides). The cDNA has 769 base pairs, the protein has 172 amino acids, and the predicted signal peptide has 18 amino acids with two cysteines. This gene is similar to I-FNs from sea bass and other teleosts. 2C I-IFN has 5 exons and 4 introns, also similar to other teleost I-IFNs. Immunohistochemical (IHC) analysis indicated that expression is predominantly membrane-localized in healthy grouper, but has a zonal distribution in nodavirus-infected grouper. Grouper infected with nodavirus had elevated levels of 2C I-IFN at 72. h and Mx at days 6-7. Recombinant 2C I-IFN activated grouper Mx, leading to upregulated antiviral activity. The grouper Mx promoter was highly induced after treatment with recombinant 2C I-IFN. The present results suggest that expression of grouper 2C I-IFN may participate in the immunologic barrier function against nodavirus.

原文English
頁(從 - 到)117-128
頁數12
期刊Developmental and Comparative Immunology
46
發行號2
DOIs
出版狀態Published - 2014 一月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

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