GeSUT4 mediates sucrose import at the symbiotic interface for carbon allocation of heterotrophic Gastrodia elata (Orchidaceae)

Li Hsuan Ho, Yung I. Lee, Shu Ying Hsieh, I. Shiuan Lin, Yun Chien Wu, Han Yu Ko, Patrick A. Klemens, H. Ekkehard Neuhaus, Yi Min Chen, Tzu Pi Huang, Chih Hsin Yeh, Woei Jiun Guo

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

Gastrodia elata, a fully mycoheterotrophic orchid without photosynthetic ability, only grows symbiotically with the fungus Armillaria. The mechanism of carbon distribution in this mycoheterotrophy is unknown. We detected high sucrose concentrations in all stages of Gastrodia tubers, suggesting sucrose may be the major sugar transported between fungus and orchid. Thick symplasm-isolated wall interfaces in colonized and adjacent large cells implied involvement of sucrose importers. Two sucrose transporter (SUT)-like genes, GeSUT4 and GeSUT3, were identified that were highly expressed in young Armillaria-colonized tubers. Yeast complementation and isotope tracer experiments confirmed that GeSUT4 functioned as a high-affinity sucrose-specific proton-dependent importer. Plasma-membrane/tonoplast localization of GeSUT4-GFP fusions and high RNA expression of GeSUT4 in symbiotic and large cells indicated that GeSUT4 likely functions in active sucrose transport for intercellular allocation and intracellular homeostasis. Transgenic Arabidopsis overexpressing GeSUT4 had larger leaves but were sensitive to excess sucrose and roots were colonized with fewer mutualistic Bacillus, supporting the role of GeSUT4 in regulating sugar allocation. This is not only the first documented carbon import system in a mycoheterotrophic interaction but also highlights the evolutionary importance of sucrose transporters for regulation of carbon flow in all types of plant-microbe interactions.

原文English
頁(從 - 到)20-33
頁數14
期刊Plant, Cell and Environment
44
發行號1
DOIs
出版狀態Published - 2021 1月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學

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