Glioblastoma cells labeled by robust Raman tags for enhancing imaging contrast

Li Ching Huang, Yung Ching Chang, Yi Syuan Wu, Wei Lun Sun, Chan Chuan Liu, Chun I. Sze, Shiuan Yeh Chen

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)


Complete removal of a glioblastoma multiforme (GBM), a highly malignant brain tumor, is challenging due to its infiltrative characteristics. Therefore, utilizing imaging agents such as fluorophores to increase the contrast between GBM and normal cells can help neurosurgeons to locate residual cancer cells during image guided surgery. In this work, Raman tag based labeling and imaging for GBM cells in vitro is described and evaluated. The cell membrane of a GBM adsorbs a substantial amount of functionalized Raman tags through overexpression of the epidermal growth factor receptor (EGFR) and “broadcasts” stronger pre-defined Raman signals than normal cells. The average ratio between Raman signals from a GBM cell and autofluorescence from a normal cell can be up to 15. In addition, the intensity of these images is stable under laser illuminations without suffering from the severe photobleaching that usually occurs in fluorescent imaging. Our results show that labeling and imaging GBM cells via robust Raman tags is a viable alternative method to distinguish them from normal cells. This Raman tag based method can be used solely or integrated into an existing fluorescence system to improve the identification of infiltrative glial tumor cells around the boundary, which will further reduce GBM recurrence. In addition, it can also be applied/extended to other types of cancer to improve the effectiveness of image guided surgery.

頁(從 - 到)2142-2153
期刊Biomedical Optics Express
出版狀態Published - 2018 5月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 原子與分子物理與光學


深入研究「Glioblastoma cells labeled by robust Raman tags for enhancing imaging contrast」主題。共同形成了獨特的指紋。