Glycan array on aluminum oxide-coated glass slides through phosphonate chemistry

Shih Huang Chang, Jeng Liang Han, Susan Y. Tseng, Hsin Yu Lee, Chin Wei Lin, Yu Chen Lin, Wen Yih Jeng, Andrew H.J. Wang, Chung Yi Wu, Chi Huey Wong

研究成果: Article同行評審

55 引文 斯高帕斯(Scopus)

摘要

A new type of glycan array covalently or noncovalently attached to aluminum oxide-coated glass (ACG) slides has been developed for studies of enzymatic reactions and protein binding. To prepare the noncovalent array, glycans with a polyfluorinated hydrocarbon (-C8F17) tail are spotted robotically onto the ACG slide surface containing a layer of polyfluorinated hydrocarbon terminated with phosphonate. After incubation and washing, the noncovalent array can be characterized by MS-TOF via ionization/desorption at a low laser energy without addition of matrix. A representive cellotetraose array was developed to study the activity and specificity of different cellulases and to differentiate the exo-and endoglucanase activities. To prepare the covalent array, glycans with a phosphonic acid tail were synthesized and spotted robotically onto the ACG slide surface. After incubation, the slides can be used directly for quantitative protein binding analysis. Compared to the preparation of glycan arrays on glass slides and other surfaces, this method of arraying using phosphonic acid reacting with ACG is more direct, convenient, and effective and represents a new platform for the high-throughput analysis of protein-glycan interactions.

原文English
頁(從 - 到)13371-13380
頁數10
期刊Journal of the American Chemical Society
132
發行號38
DOIs
出版狀態Published - 2010 9月 29

All Science Journal Classification (ASJC) codes

  • 催化
  • 一般化學
  • 生物化學
  • 膠體和表面化學

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