@article{02c64c8189b64bea947e01fe4085758b,
title = "Glycan-binding preferences and genetic evolution of human seasonal influenza A (H3N2) viruses during 1999-2007 in Taiwan",
abstract = "It is generally agreed that human influenza virus preferentially binds to α-2,6-linked sialic acid-containing receptors, and mutations that change the binding preference may alter virus infectivity and host tropism. Limited information is available on the glycan-binding specificity of epidemic influenza viruses. In this study, we systemically investigated the glycan-binding preferences of human influenza A(H3N2) viruses isolated from 1999 to 2007 in Taiwan using a high-throughput carbohydrate array. The binding patterns of 37 H3N2 viruses were classified into three groups with significant binding-pattern variations. The results showed that the carbohydrate-binding patterns of H3N2 varied over time. A phylogenetic analysis of the hemagglutinin gene also revealed progressive drift year to year. Of note, the viruses that caused large outbreaks in 1999 and 2003 showed glycan-binding preferences to both α-2,3 and α-2,6 sialylated glycans. Twenty amino acid substitutions were identified primarily at antigenic sites that might contribute to H3N2 virus evolution and the change in the glycan-binding patterns. This study provides not only a systematic analysis of the receptor-binding specificity of influenza clinical isolates but also information that could help to monitor the outbreak potential and virus evolution of influenza viruses.",
author = "Wang, {Ya Fang} and Chang, {Chuan Fa} and Tsai, {Huey Pin} and Chi, {Chia Yu} and Su, {Ih Jen} and Wang, {Jen Ren}",
note = "Funding Information: This study was financially supported by grants from the National Health Research Institutes (99A1-IDPP13-014, 05A1-IVPP09-014), Center of Infectious Diseases and Signal Research, National Cheng Kung University, Aim for the Top University Project, Ministry of Education, and Ministry of Science and Technology (MOST) (grant no. MOST 104-2321-B006-002). The authors thank Ching-Hui Tai for technical assistance in this study. This study was financially supported by grants from the National Health Research Institutes (grant nos. 99A1-IDPP13-014 and 05A1-IVPP09-014), the Center of Infectious Diseases and Signal Research, National Cheng Kung University, the Aim for the Top University Project, the Ministry of Education, and the Ministry of Science and Technology (MOST) (grant no. MOST 104-2321-B006-002). Publisher Copyright: {\textcopyright} 2018 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2018",
month = may,
doi = "10.1371/journal.pone.0196727",
language = "English",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",
}