Green tea catechin (-)-Epigallocatechin-3-gallate (EGCG) facilitates fracture healing

Sung Yen Lin, Jung Yu Kan, Cheng Chang Lu, Han Hsiang Huang, Tsung Lin Cheng, Hsuan Ti Huang, Cheng Jung Ho, Tien Ching Lee, Shu Chun Chuang, Yi Shan Lin, Lin Kang, Chung Hwan Chen

研究成果: Article

摘要

Green tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGCG can also enhance the osteogenic differentiation of murine bone marrow mesenchymal stem cells and inhibit the osteoclastogenesis in RAW264.7 cells by modulation of the receptor activator of nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegrin (OPG) (RANK/RANKL/OPG) pathway. Our previous study also found that EGCG can promote bone defect healing in the distal femur partially via bone morphogenetic protein-2 (BMP-2). Considering the osteoinduction property of BMP-2, we hypothesized that EGCG could accelerate the bone healing process with an increased expression of BMP-2. In this manuscript, we studied whether the local use of EGCG can facilitate tibial fracture healing. Fifty-six 4-month-old rats were randomly assigned to two groups after being weight-matched: A control group with vehicle treatment (Ctrl) and a study group with 10 μmol/L, 40 μL, EGCG treatment (EGCG). Two days after the operation, the rats were treated daily with EGCG or vehicle by percutaneous local injection for 2 weeks. The application of EGCG enhanced callus formation by increasing the bone volume and subsequently improved the mechanical properties of the tibial bone, including the maximal load, break load, stiffness, and Young's modulus. The results of the histology and BMP-2 immunohistochemistry staining showed that EGCG treatment accelerated the bone matrix formation and produced a stronger expression of BMP-2. Taken together, this study for the first time demonstrated that local treatment of EGCG can accelerate the fracture healing process at least partly via BMP-2.

原文English
文章編號620
期刊Biomolecules
10
發行號4
DOIs
出版狀態Published - 2020 四月

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Lin, S. Y., Kan, J. Y., Lu, C. C., Huang, H. H., Cheng, T. L., Huang, H. T., Ho, C. J., Lee, T. C., Chuang, S. C., Lin, Y. S., Kang, L., & Chen, C. H. (2020). Green tea catechin (-)-Epigallocatechin-3-gallate (EGCG) facilitates fracture healing. Biomolecules, 10(4), [620]. https://doi.org/10.3390/biom10040620