TY - JOUR
T1 - Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern
AU - Ding, Jiun Yan
AU - Chen, Sheng Chung
AU - Lai, Mei Chin
AU - Liao, Tsai Ling
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Taiwan, ROC under the ATU plan (to M. C. L.), and the Ministry of Science and Technology, Taiwan, ROC (MOST103-3113 M-005-001, MOST 104-3113 M-005-001 and MOST105-3113 M-005-001).
Publisher Copyright:
© 2017 IUMS.
PY - 2017/5
Y1 - 2017/5
N2 - A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10μm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51%; rpoB′ sequence similarity: 96.19%) and Haloterrigena thermotolerans PR5T (99.11%; 95.50%). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68%, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00%, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).
AB - A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10μm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51%; rpoB′ sequence similarity: 96.19%) and Haloterrigena thermotolerans PR5T (99.11%; 95.50%). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68%, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00%, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).
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U2 - 10.1099/ijsem.0.001811
DO - 10.1099/ijsem.0.001811
M3 - Article
C2 - 28109205
AN - SCOPUS:85020448586
VL - 67
SP - 1333
EP - 1338
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 5
M1 - 001811
ER -