Hard clam extracts induce atypical apoptosis in human gastric cancer cells

Eing Ju Song, Michael W.Y. Chan, Jyh Wei Shin, Che Chun Chen

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Hard clams (HCs) are a nutritionally high-quality and popular seafood, and are established to be a potent antitumor food. The aim of the present study was to determine whether HC extracts induce apoptosis in the human gastric cancer cell line, AGS. In contrast with previously reported methods of extraction, crude extracts of HC were obtained by freezing and thawing and by a method free of hot water or organic solvents. The composition, quality and properties of the HC extracts were demonstrated to be stable since the extracts that were evaluated by capillary electrophoresis and HPLC analysis at different timepoints were similar. HC extracts also have an inhibitory effect against the survival of AGS cells. Treatment with HC extracts induced a marked sub-G1 DNA peak and reduced the expression of the anti-apoptotic genes BIRC5 and KPNA2. However, hallmarks of classical apoptosis such as DNA fragmentation and apoptotic body formation were not observed, indicating atypical apoptosis. Furthermore, it was revealed that HC extracts interrupted cell cycle progression in AGS cells through altered expression of six cell cycle-associated genes: CDC20, KPNA2, BIRC5, ANAPC2, CDKN1A and RB1. The present findings suggest that HC may contribute to a novel future anticancer agent.

原文English
頁(從 - 到)1409-1418
頁數10
期刊Experimental and Therapeutic Medicine
14
發行號2
DOIs
出版狀態Published - 2017 八月

All Science Journal Classification (ASJC) codes

  • 免疫學與微生物學(雜項)
  • 癌症研究

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