Hemodynamic flow visualization of early embryonic great vessels using μPIV

Selda Goktas, Chia Yuan Chen, William J. Kowalski, Kerem Pekkan

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Microparticle image velocimetry (μPIV) is an evolving quantitative methodology to closely and accurately monitor the cardiac flow dynamics and mechanotransduction during vascular morphogenesis. While PIV technique has a long history, contemporary developments in advanced microscopy have significantly expanded its power. This chapter includes three new methods for μPIV acquisition in selected embryonic structures achieved through advanced optical imaging: (1) high-speed confocal scanning of transgenic zebrafish embryos, where the transgenic erythrocytes act as the tracing particles; (2) microinjection of artificial seeding particles in chick embryos visualized with stereomicroscopy; and (3) real-time, timeresolved optical coherence tomography acquisition of vitelline vessel flow profiles in chick embryos, tracking the erythrocytes.

原文English
頁(從 - 到)17-30
頁數14
期刊Methods in Molecular Biology
1189
DOIs
出版狀態Published - 2015

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學

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