Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA

Chao Liang Wu, Tzong Shiann Leu, Ting Tsung Chang, Ai Li Shiau

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

The sequence encoding the truncated core protein (amino acids 1-98) of hepatitis C virus (HCc) was expressed in E. coli production of HCc((1-98)), or fused with the truncated core antigen (HBcAg) and segments from the preS1 and preS2 regions from hepatitis B virus (HBV) for production of HBcPreS1PreS2HCc((1-98)). The HCc((1-98)) and HBcPreS1PreS2HCc((1-98)) proteins reacted with sera from HCV-infected individuals by immunoblot analyses, while the latter protein also exhibited HBV core antigenicity. They induced antibodies against HBcAg and/or HCV core protein in rabbits and in mice. Moreover, HBcPreS1PreS2HCc((1-98)) is more immunogenic than HCc((1- 98)) in terms of anti-HCc induction. An ELISA that employed recombinant HCV core antigens of either HCc((1-98)) or HBcPreS1PreS2HCc((1-98)) to detect anti-HCc and/or anti-HBc antibodies was developed. Evaluation of serum samples with different status of HBV and HCV infections suggested that HCc((1-98)) might be suitable for the determination of antibodies against HCV core protein, while HBcPreS1PreS2HCc((1-98)) might be of value to detect HCV and/or HBV infection in donated blood in HBV low-prevalence countries.

原文English
頁(從 - 到)104-110
頁數7
期刊Journal of Medical Virology
57
發行號2
DOIs
出版狀態Published - 1999 1月 14

All Science Journal Classification (ASJC) codes

  • 病毒學
  • 傳染性疾病

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