Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine

Chun Yen Chu; Shiang Yiu Wang; Zeng Weng Chen; Maw Sheng Chien; Ji Ping Huang; Jen Jin Chen; Li Shian Hong; Ai Li Shiau; Jeng Liang Tsai; Chao Liang Wu

doi:10.1016/j.vaccine.2007.07.063

Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine

Chun Yen Chu, Shiang Yiu Wang, Zeng Weng Chen, Maw Sheng Chien, Ji Ping Huang, Jen Jin Chen, Li Shian Hong, Ai Li Shiau, Jeng Liang Tsai, Chao Liang Wu

研究成果: Article › 同行評審

11 引文斯高帕斯（Scopus）

摘要

In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Δcrp/vpl^-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Δcrp/vpl^- live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Δcrp/vpl^- can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.

原文	English
頁（從 - 到）	7031-7040
頁數	10
期刊	Vaccine
卷	25
發行號	41
DOIs	https://doi.org/10.1016/j.vaccine.2007.07.063
出版狀態	Published - 2007 十月 10

All Science Journal Classification (ASJC) codes

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

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title = "Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine",

abstract = "In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Δcrp/vpl- live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Δcrp/vpl- can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.",

author = "Chu, {Chun Yen} and Wang, {Shiang Yiu} and Chen, {Zeng Weng} and Chien, {Maw Sheng} and Huang, {Ji Ping} and Chen, {Jen Jin} and Hong, {Li Shian} and Shiau, {Ai Li} and Tsai, {Jeng Liang} and Wu, {Chao Liang}",

note = "Funding Information: This study was supported by the SBIR promotion program IZ920114 of Ministry of Economic Affairs, Taiwan. The authors thank Dr. Tsung-Chou Chang (Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan) for kindly helping pathological examination of pigs. ",

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Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine. / Chu, Chun Yen; Wang, Shiang Yiu; Chen, Zeng Weng; Chien, Maw Sheng; Huang, Ji Ping; Chen, Jen Jin; Hong, Li Shian; Shiau, Ai Li; Tsai, Jeng Liang; Wu, Chao Liang.

於: Vaccine, 卷 25, 編號 41, 10.10.2007, p. 7031-7040.

研究成果: Article › 同行評審

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AU - Chu, Chun Yen

AU - Wang, Shiang Yiu

AU - Chen, Zeng Weng

AU - Chien, Maw Sheng

AU - Huang, Ji Ping

AU - Chen, Jen Jin

AU - Hong, Li Shian

AU - Shiau, Ai Li

AU - Tsai, Jeng Liang

AU - Wu, Chao Liang

N1 - Funding Information: This study was supported by the SBIR promotion program IZ920114 of Ministry of Economic Affairs, Taiwan. The authors thank Dr. Tsung-Chou Chang (Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan) for kindly helping pathological examination of pigs.

PY - 2007/10/10

Y1 - 2007/10/10

N2 - In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Δcrp/vpl- live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Δcrp/vpl- can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.

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