High levels of regulatory T cells in blood are a poor prognostic factor in patients with diffuse large B-cell lymphoma

Chen Chang, Shang Yin Wu, Yu Wei Kang, Kun Piao Lin, Tsai Yun Chen, L. Jeffrey Medeiros, Kung Chao Chang

研究成果: Article

11 引文 (Scopus)

摘要

Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), "natural" regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and "induced" Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.

原文English
頁(從 - 到)935-944
頁數10
期刊American Journal of Clinical Pathology
144
發行號6
DOIs
出版狀態Published - 2015 十二月

指紋

Lymphoma, Large B-Cell, Diffuse
Regulatory T-Lymphocytes
Dendritic Cells
Healthy Volunteers
Neutrophils
Lymphocytes
Immunophenotyping
CD4-CD8 Ratio
T-Lymphocyte Subsets
Adaptive Immunity
Helper-Inducer T-Lymphocytes
Leukocyte Count
Monocytes
Immunity
Blood Cells
Flow Cytometry
Multivariate Analysis
Survival

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

引用此文

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title = "High levels of regulatory T cells in blood are a poor prognostic factor in patients with diffuse large B-cell lymphoma",
abstract = "Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), {"}natural{"} regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and {"}induced{"} Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.",
author = "Chen Chang and Wu, {Shang Yin} and Kang, {Yu Wei} and Lin, {Kun Piao} and Chen, {Tsai Yun} and Medeiros, {L. Jeffrey} and Chang, {Kung Chao}",
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T1 - High levels of regulatory T cells in blood are a poor prognostic factor in patients with diffuse large B-cell lymphoma

AU - Chang, Chen

AU - Wu, Shang Yin

AU - Kang, Yu Wei

AU - Lin, Kun Piao

AU - Chen, Tsai Yun

AU - Medeiros, L. Jeffrey

AU - Chang, Kung Chao

PY - 2015/12

Y1 - 2015/12

N2 - Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), "natural" regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and "induced" Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.

AB - Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), "natural" regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and "induced" Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.

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