High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and-3 expression via CD44 in tendinopathy

Po Ting Wu, Li Chieh Kuo, Fong Chin Su, Shih Yao Chen, Tai I. Hsu, Chung Yi Li, Kuen Jer Tsai, I. Ming Jou

研究成果: Article

7 引文 (Scopus)

摘要

Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350 kDa, 1500 kDa, and 3000 kDa) on matrix metalloproteinase (MMP)-1 and-3 expression in IL-1β-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Reverse transcription PCR, real-time PCR, and ELISA were used to determine MMP-1 and-3expression. Because CD44 was clearly expressed in the plasma membranes of cultured tenocytes, OX-50, a CD44 antagonist, was used to inhibit CD44 to evaluate the HA mechanism. HA (3000 kDa) significantly (p < 0.001) downregulated the mRNA and protein expression of MMP-1 and-3 in IL-1β-stimulated tenocytes. Its attenuating effects were dose-dependent (p < 0.01). In OX-50-pretreated cells, the mRNA expression of CD44 was not significantly altered, but the mRNA expression of MMP-1 and-3 was significantly upregulated. Visual analogue scale scores were significantly lower, and MMP-1 and-3 expression was significantly (p < 0.05) lower one month posttreatment. HMW-HA attenuated tendinopathy by downregulating MMP-1 and-3 expression. Inhibiting CD44 blocked the effects of HMW-HA.

原文English
文章編號40840
期刊Scientific reports
7
DOIs
出版狀態Published - 2017 一月 16

指紋

Matrix Metalloproteinase 3
Tendinopathy
Matrix Metalloproteinase 1
Hyaluronic Acid
Molecular Weight
Interleukin-1
Messenger RNA
Down-Regulation
Visual Analog Scale
Reverse Transcription
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Cell Membrane
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • General

引用此文

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title = "High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and-3 expression via CD44 in tendinopathy",
abstract = "Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350 kDa, 1500 kDa, and 3000 kDa) on matrix metalloproteinase (MMP)-1 and-3 expression in IL-1β-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Reverse transcription PCR, real-time PCR, and ELISA were used to determine MMP-1 and-3expression. Because CD44 was clearly expressed in the plasma membranes of cultured tenocytes, OX-50, a CD44 antagonist, was used to inhibit CD44 to evaluate the HA mechanism. HA (3000 kDa) significantly (p < 0.001) downregulated the mRNA and protein expression of MMP-1 and-3 in IL-1β-stimulated tenocytes. Its attenuating effects were dose-dependent (p < 0.01). In OX-50-pretreated cells, the mRNA expression of CD44 was not significantly altered, but the mRNA expression of MMP-1 and-3 was significantly upregulated. Visual analogue scale scores were significantly lower, and MMP-1 and-3 expression was significantly (p < 0.05) lower one month posttreatment. HMW-HA attenuated tendinopathy by downregulating MMP-1 and-3 expression. Inhibiting CD44 blocked the effects of HMW-HA.",
author = "Wu, {Po Ting} and Kuo, {Li Chieh} and Su, {Fong Chin} and Chen, {Shih Yao} and Hsu, {Tai I.} and Li, {Chung Yi} and Tsai, {Kuen Jer} and Jou, {I. Ming}",
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AU - Wu, Po Ting

AU - Kuo, Li Chieh

AU - Su, Fong Chin

AU - Chen, Shih Yao

AU - Hsu, Tai I.

AU - Li, Chung Yi

AU - Tsai, Kuen Jer

AU - Jou, I. Ming

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N2 - Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350 kDa, 1500 kDa, and 3000 kDa) on matrix metalloproteinase (MMP)-1 and-3 expression in IL-1β-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Reverse transcription PCR, real-time PCR, and ELISA were used to determine MMP-1 and-3expression. Because CD44 was clearly expressed in the plasma membranes of cultured tenocytes, OX-50, a CD44 antagonist, was used to inhibit CD44 to evaluate the HA mechanism. HA (3000 kDa) significantly (p < 0.001) downregulated the mRNA and protein expression of MMP-1 and-3 in IL-1β-stimulated tenocytes. Its attenuating effects were dose-dependent (p < 0.01). In OX-50-pretreated cells, the mRNA expression of CD44 was not significantly altered, but the mRNA expression of MMP-1 and-3 was significantly upregulated. Visual analogue scale scores were significantly lower, and MMP-1 and-3 expression was significantly (p < 0.05) lower one month posttreatment. HMW-HA attenuated tendinopathy by downregulating MMP-1 and-3 expression. Inhibiting CD44 blocked the effects of HMW-HA.

AB - Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350 kDa, 1500 kDa, and 3000 kDa) on matrix metalloproteinase (MMP)-1 and-3 expression in IL-1β-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Reverse transcription PCR, real-time PCR, and ELISA were used to determine MMP-1 and-3expression. Because CD44 was clearly expressed in the plasma membranes of cultured tenocytes, OX-50, a CD44 antagonist, was used to inhibit CD44 to evaluate the HA mechanism. HA (3000 kDa) significantly (p < 0.001) downregulated the mRNA and protein expression of MMP-1 and-3 in IL-1β-stimulated tenocytes. Its attenuating effects were dose-dependent (p < 0.01). In OX-50-pretreated cells, the mRNA expression of CD44 was not significantly altered, but the mRNA expression of MMP-1 and-3 was significantly upregulated. Visual analogue scale scores were significantly lower, and MMP-1 and-3 expression was significantly (p < 0.05) lower one month posttreatment. HMW-HA attenuated tendinopathy by downregulating MMP-1 and-3 expression. Inhibiting CD44 blocked the effects of HMW-HA.

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