High-throughput fabrication of gray-level biomicrostructures via temporal focusing excitation and laser pulse control

Yi Cheng Li, Li Chung Cheng, Chia Yuan Chang, Chun Yu Lin, Nan Shan Chang, Paul J. Campagnola, Chen Yuan Dong, Shean Jen Chen

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

A developed temporal focusing-based multiphoton excitation system with additional patterned excitation and local laser control can provide high-throughput fabrication of three-dimensional gray-level biomicrostructures via two-photon cross-linking with rose bengal (RB) as the photoactivator. Multiple bovine serum albumin (BSA) structures of different concentrations were simultaneously achieved by selecting different pulse numbers in the designated regions with an appropriate femtosecond laser power within a few seconds. Intensity of the RB two-photon excited fluorescence (TPEF) is correlated to the concentration of the fabricated cross-linked BSA microstructure. Hence, the fabricated BSA microstructure can be monitored online by utilizing the RB TPEF as contrast agent. It suggests that this approach with its unique capability of high-speed, gray-level, and online-inspection fabrication meets the requirements of the biomedical researches involved in extracellular matrix.

原文English
文章編號075004
期刊Journal of Biomedical Optics
18
發行號7
DOIs
出版狀態Published - 2013 7月

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 原子與分子物理與光學
  • 生物材料
  • 生物醫學工程

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