HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma

Ching Ling Cheng, Ya Win Chang, Chen-Hsi Chou

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.

原文English
頁(從 - 到)281-288
頁數8
期刊Biomedical Chromatography
24
發行號3
DOIs
出版狀態Published - 2010 三月 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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