Human papilloma virus detection in neoplastic and non-neoplastic nasopharyngeal tissues in Taiwan

Cheng Chih Huang, Jenn Ren Hsiao, Ming Wei Yang, Yuan Hua Wu, Keng Fu Hsu, Yao Chang, Chaio Wei Chen, Sen Tien Tsai, Hsuan Pei Wei, Ying Tai Jin

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

Background: Human papilloma virus (HPV) has been implicated in the carcinogenesis and prognosis of certain head and neck cancers. Whether it also has a role in the pathogenesis of nasopharyngeal carcinoma (NPC) in Taiwan is unclear. Methods: Detection and genotyping of HPVs were performed in 43 primary NPCs (one WHO-I and 42 WHO-II/III) and 40 nasopharyngeal controls using PCR-based HPV genotyping arrays. Localisation of high-risk HPV and Epstein-Barr virus genomes was performed in another 46 primary NPCs (five WHO-I and 41 WHO-II/III) and seven paired metastatic WHO-II/III NPCs using in situ hybridisation. Results: In the HPV genotyping cohort, oncogenic HPVs were detected equally in WHO-II/III NPCs (31%, 13/42) and nasopharyngeal controls (35%, 14/40). Tumour high-risk HPV status did not correlate with the prognosis of patients with NPC. In the high-risk HPV in situ hybridisation cohort, 14 (88%) of the 16 oncogenic HPV-positive WHO-II/III NPCs showed a unique cytoplasmic/perinuclear staining pattern, which is distinct from the typical dot/punctate nuclear staining pattern indicating HPV genome integration. In addition, oncogenic HPVs were not always retained in NPC cells during the process of metastasis. Conclusions: This study does not support an association between oncogenic HPV and the carcinogenesis or prognosis of WHO-II/III NPCs in Taiwan.

原文English
頁(從 - 到)571-577
頁數7
期刊Journal of Clinical Pathology
64
發行號7
DOIs
出版狀態Published - 2011 7月

All Science Journal Classification (ASJC) codes

  • 病理學與法醫學

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