Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells

Po Yang Chen, Lynn Ling-Huei Huang, Hsyue Jen Hsieh

研究成果: Article

35 引文 (Scopus)

摘要

For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs.

原文English
頁(從 - 到)1-6
頁數6
期刊Biochemical and Biophysical Research Communications
360
發行號1
DOIs
出版狀態Published - 2007 八月 17

指紋

Hyaluronic Acid
Stromal Cells
Control surfaces
Culture Media
Cell Aging
Osteogenesis
Cell Culture Techniques
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

引用此文

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