Hydroxyapatite formation with the interface of chitin and chitosan

Chen Hsueh Lin, Yu Sheng Chen, Wei Lun Huang, Tzung Chin Hung, Ten Chin Wen

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)


Hydroxyapatite (HA) film atop artificial implants surface can provide bioactive, osteoconductive and bone-similar interface. Traditional technique utilizes collagen coating to induce HA mineralization via charged functional groups. However, collagen loss as aging might lead to unstable interface. In this study, we presume that chitin (CH) with acetamide group chelates calcium ion and chitosan (CS) with amine group chelates phosphate ion, providing an ambiance for the HA crystallite formation. The presumption is evidenced by molar conductivity experiments. Besides, the hydrogen bonds of CH and electrostatic repulsion force of CS render different molecular architecture for the tunnel-connected structure. Furthermore, it forms porous morphology after HA mineralization evidenced by SEM images. Interestingly, mixing CH and CS provides the interface for Ca2+/PO43- capturing, rendering the formation of micro-crystallite with the 1.69/1.7 for the ratio of calcium to phosphate, being evidenced by EDX and AES experiments. Moreover, crystallinity index is calculated with XRD and FTIR spectra that CH–CS–HA possesses the highest crystallite to amorphous ratio. The HA film also shows good biocompatibility through L929 MTT test with 100% cell viability for the potential bio-applications. By the tapping-mode of AFM, the Young's modulus of CH–CS–HA surface is estimated to be 2.04 GPa near that of native bone.

頁(從 - 到)294-300
期刊Journal of the Taiwan Institute of Chemical Engineers
出版狀態Published - 2021 1月

All Science Journal Classification (ASJC) codes

  • 一般化學
  • 一般化學工程


深入研究「Hydroxyapatite formation with the interface of chitin and chitosan」主題。共同形成了獨特的指紋。