TY - JOUR
T1 - Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba
AU - Huang, Jian Ming
AU - Chang, Yao Tsung
AU - Shih, Min Hsiu
AU - Lin, Wei Chen
AU - Huang, Fu Chin
N1 - Funding Information:
Funding This research was supported by the Ministry of Science and Technology (MOST) to WCL (grant MOST 106-2320-B-006-070-MY3) and by the National Cheng Kung University Hospital to FCH.
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Acanthamoeba is a free-living pathogenic protozoan that is distributed in different environmental reservoirs, including lakes and soil. Pathogenic Acanthamoeba can cause severe human diseases, such as blinding keratitis and granulomatous encephalitis. Therefore, it is important to understand the pathogenic relationship between humans and Acanthamoeba. By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. We investigated the molecular functions and characteristics of the M28AP protein by anti-M28 antibodies and a M28AP mutant strain generated by the CRISPR/Cas9 system. Human complement proteins such as C3b and iC3b were degraded by Acanthamoeba M28AP. We believe that M28AP is an important factor in human innate immunity. This study provides new insight for the development of more efficient medicines to treat Acanthamoeba infection.
AB - Acanthamoeba is a free-living pathogenic protozoan that is distributed in different environmental reservoirs, including lakes and soil. Pathogenic Acanthamoeba can cause severe human diseases, such as blinding keratitis and granulomatous encephalitis. Therefore, it is important to understand the pathogenic relationship between humans and Acanthamoeba. By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. We investigated the molecular functions and characteristics of the M28AP protein by anti-M28 antibodies and a M28AP mutant strain generated by the CRISPR/Cas9 system. Human complement proteins such as C3b and iC3b were degraded by Acanthamoeba M28AP. We believe that M28AP is an important factor in human innate immunity. This study provides new insight for the development of more efficient medicines to treat Acanthamoeba infection.
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U2 - 10.1007/s00436-019-06332-8
DO - 10.1007/s00436-019-06332-8
M3 - Article
C2 - 31065830
AN - SCOPUS:85065461792
SN - 0932-0113
VL - 118
SP - 1865
EP - 1874
JO - Parasitology Research
JF - Parasitology Research
IS - 6
ER -
