Identification and characterization of epitopes on Cyn d I, the major allergen of Bermuda grass pollen

Shou Hwa Han, Zo Nan Chang, Hwei Hwa Chang, Chin Wen Chi, Jiu Yao Wang, Ching Yuang Lin

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Background: We identified three epitopes on Cyn d I by using four anti-Cyn d I monoclonal antibodies (MoAbs). Methods: In a cross-inhibition binding assay, the binding of MoAbs 1-61 and 10-7 to Cyn d I was completely blocked by each other but not by MoAbs 4-37 and 11-7; the binding of MoAb 4-37 and MoAb 11-7 to Cyn d I was inhibited by themselves but not by other MoAbs. The epitope recognized by MoAbs 1-61 and 10-7 is designated as El, and those recognized by MoAbs 4-37 and 11-7 are designated as E2 and E3, respectively. Results: In a radioallergosorbent inhibition assay, we found that MoAbs 1-61 and 4-37 (1:50 diluted) can inhibit the binding of human Immunoglobulin Es to Cyn d I by more than 30%, whereas MoAb I1-7 was less efficient (reduced by only 6%). These results suggest that both El and E2 are major allergenic epitopes but that E3 is only a minor one. Further characterization of E1 and E2 reveals that they are labile in alkaline but resistant to acid and sodium periodate treatments. Moreover, E1 is heat-labile, but guanidine- and urea-sensitive, whereas E2 is not. Both E1 and E2 lost their antigenicity after reduction and alkylation. Conclusions: Results of the present study provide important information on the physicochemical properties of major allergenic epitopes on Cyn d 1, which may be useful for future development of therapeutic peptides for patients allergic to Bermuda grass pollen.

原文English
頁(從 - 到)1035-1041
頁數7
期刊The Journal of Allergy and Clinical Immunology
91
發行號5
DOIs
出版狀態Published - 1993 五月

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 免疫學

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