Identification of early intestinal neoplasia protein biomarkers using laser capture microdissection and MALDI MS

Baogang J. Xu, Jiaqing Li, R. Daniel Beauchamp, Yu Shyr, Ming Li, M. Kay Washington, Timothy J. Yeatman, Robert H. Whitehead, Robert J. Coffey, Richard M. Caprioli

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)


Obtaining protein profiles from a homogeneous cell population in tissues can significantly improve our capability in protein biomarker discovery. In this study, unique protein profiles from the top and bottom sections of mouse crypts and ApcMin+/- adenomas were obtained using laser capture microdissection (LCM) combined with MALDI MS. Statistically significant protein peaks with differential expression were selected, and a set of novel protein biomarkers were identified. Immunohistochemistry was performed to confirm the differentially expressed protein biomarkers found by LCM combined with MALDI MS. To validate the relevance of the findings in human colorectal cancer (CRC), S100A8 was further confirmed in human CRC using immunohistochemistry. In addition, S100A8 was found to have an increased expression at different human CRC stages (Duke's A-D) compared with controls at both protein (n = 168 cases) and RNA (n = 215 cases) levels. Overall LCM combined with MALDI MS is a promising method to identify intestinal protein biomarkers from minute amounts of tissue. The novel protein biomarkers identified from the top and bottom crypts will increase our knowledge of the specific protein changes taking place during cell migration from the crypt bottom to top. In addition, the identified cancer protein biomarkers will aid in the exploration of colorectal tumorigenesis mechanisms as well as in the advancement of molecularly based diagnosis of colorectal cancer.

頁(從 - 到)936-945
期刊Molecular and Cellular Proteomics
出版狀態Published - 2009 5月

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 分子生物學


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