Identification of putative miRNAs from the deep-branching unicellular flagellates

Po Jung Huang, Wei Chen Lin, Shih Chieh Chen, Yong Han Lin, Chin Hung Sun, Ping Chiang Lyu, Petrus Tang

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)


MicroRNAs (miRNAs) are a class of extensively studied RNAi-associated small RNAs that play a critical role in eukaryotic gene regulation. However, knowledge on the miRNA and its regulation in unicellular eukaryotes is very limited. In order to obtain a better understanding on the origin of miRNA regulation system, we used deep-sequencing technology to investigate the miRNA expression pattern in four deep-branching unicellular flagellates: Giardia lamblia, Trichomonas vaginalis, Tritrichomonas foetus, and Pentatrichomonas hominis. In addition to the known miRNAs that have been described in G. lamblia and T. vaginalis, we identified 14 ancient animal miRNA families and 13 plant-specific families. Bioinformatics analysis also identified four novel miRNA candidates with reliable precursor structures derived from mature tRNAs. Our results indicated that miRNAs are likely to be a general feature for gene regulation throughout unicellular and multicellular eukaryotes and some of them may derive from unconventional ncRNAs such as snoRNA and tRNA.

頁(從 - 到)101-107
出版狀態Published - 2012 二月

All Science Journal Classification (ASJC) codes

  • 遺傳學


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