TY - JOUR
T1 - Identification of Staphylococcus spp. and detection of mecA by an oligonucleotide array
AU - Han, Huan Wen
AU - Chang, Hsien Chang
AU - Chang, Tsung Chain
N1 - Funding Information:
Financial support: This project was supported by grants from the Ministry of Science and Technology (MOST 102-2320-B-026-MY3) and the Multidisciplinary Center of Excellence for Clinical Trial and Research (MOHW105-TDU-B-211-133016), Department of Health and Welfare, Taiwan. These funding organizations had no role in the design or conduct of this research.
Funding Information:
This project was supported by grants from the Ministry of Science and Technology (MOST 102-2320-B-026-MY3) and the Multidisciplinary Center of Excellence for Clinical Trial and Research (MOHW105-TDU-B-211-133016), Department of Health and Welfare, Taiwan. The funding organizations had no role in the design or conduct of this research. We thank Ay-Huey Huang at the Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan, for her collection of clinical isolates.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (30 species) and 434 clinical isolates of staphylococci were analyzed. Gene sequencing of 16S rRNA, gap or tuf genes was the reference method for species identification. All reference strains (100%) were correctly identified, while the identification rates of clinical isolates of S. aureus and CoNS were 98.9% and 98%, respectively. The sensitivity and specificity for mecA detection were 99% and 100%, respectively, in S. aureus isolates, and both values were 100% in isolates of CoNS. The assay takes 6 h from a purified culture isolate, and so far it has not been performed directly on patient samples.
AB - Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (30 species) and 434 clinical isolates of staphylococci were analyzed. Gene sequencing of 16S rRNA, gap or tuf genes was the reference method for species identification. All reference strains (100%) were correctly identified, while the identification rates of clinical isolates of S. aureus and CoNS were 98.9% and 98%, respectively. The sensitivity and specificity for mecA detection were 99% and 100%, respectively, in S. aureus isolates, and both values were 100% in isolates of CoNS. The assay takes 6 h from a purified culture isolate, and so far it has not been performed directly on patient samples.
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U2 - 10.1016/j.diagmicrobio.2016.06.003
DO - 10.1016/j.diagmicrobio.2016.06.003
M3 - Article
C2 - 27342780
AN - SCOPUS:84990030124
SN - 0732-8893
VL - 86
SP - 23
EP - 29
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
ER -
