TY - JOUR
T1 - Identification of sucrose-regulated genes in cultured rice cells using mRNA differential display
AU - Tseng, Ta Chien
AU - Tsai, Teh Huei
AU - Lue, Ming Yong
AU - Lee, Hung tu
N1 - Funding Information:
We thank Dr. Li-Fei Liu for kindly providing the rice suspension cells. This work was supported by the grant NSC84-231 l-B-007-020 from the National Science Council of Taiwan, ROC.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1995/8/19
Y1 - 1995/8/19
N2 - In order to get more information about carbon metabolite regulation pathways, cloning and sequence analysis of sucrose-regulated genes from rice-suspension-cultured cells were performed. We used a new method, mRNA differential display, to screen differentially expressed genes under conditions of 3% and no sucrose in the cultured medium. Six candidate clones were identified and sequenced. Clones SI1 and SI2 were repressed by sucrose starvation, while clones SR1, SR2, SR3 and SR4 were induced by sucrose starvation. Nucleotide sequence analysis showed that clone SR2 has 94.8% homology to the salT gene, and clones SI1 and SR3 show 88.3 and 96.9% identity, respectively, to partial cDNA sequences in the GenBank database. The results suggest that mRNA differential display provides an easy and quick way to clone genes involved in the carbon metabolite regulation pathway.
AB - In order to get more information about carbon metabolite regulation pathways, cloning and sequence analysis of sucrose-regulated genes from rice-suspension-cultured cells were performed. We used a new method, mRNA differential display, to screen differentially expressed genes under conditions of 3% and no sucrose in the cultured medium. Six candidate clones were identified and sequenced. Clones SI1 and SI2 were repressed by sucrose starvation, while clones SR1, SR2, SR3 and SR4 were induced by sucrose starvation. Nucleotide sequence analysis showed that clone SR2 has 94.8% homology to the salT gene, and clones SI1 and SR3 show 88.3 and 96.9% identity, respectively, to partial cDNA sequences in the GenBank database. The results suggest that mRNA differential display provides an easy and quick way to clone genes involved in the carbon metabolite regulation pathway.
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U2 - 10.1016/0378-1119(95)00243-Y
DO - 10.1016/0378-1119(95)00243-Y
M3 - Article
C2 - 7665075
AN - SCOPUS:0028977956
VL - 161
SP - 179
EP - 182
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -