Identification of the cell fate gene stalky in dictyostelium

Wen Tsan Chang, Peter C. Newell, Julian D. Gross

研究成果: Article同行評審

46 引文 斯高帕斯(Scopus)


Using insertional mutagenesis, we have isolated a 'stalky' mutant in which cells destined to become spores end up as stalk cells. Similar mutants were previously observed after chemical mutagenesis, but the affected gene could not be isolated. Our mutant, like the previous ones, is in stkA. Its defect is cell-autonomous and not overcome by overexpressing cAMP-dependent protein kinase. stkA is strongly expressed in the prespore region of aggregates but not in the anterior prestalk zone. The mutant expresses normal levels of prespore-cell transcripts but fails to produce the spore transcript spiA. stkA encodes a predicted 99 kDa protein (STKA) with two putative C4 zinc fingers, one of which is a GATA-type finger, indicating that it may be a transcription factor. This conclusion is supported by localization of STKA in the nucleus.

頁(從 - 到)471-481
出版狀態Published - 1996 11月 1

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)


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