Identification of the cell fate gene stalky in dictyostelium

Wen Tsan Chang, Peter C. Newell, Julian D. Gross

研究成果: Article同行評審

46 引文 斯高帕斯(Scopus)

摘要

Using insertional mutagenesis, we have isolated a 'stalky' mutant in which cells destined to become spores end up as stalk cells. Similar mutants were previously observed after chemical mutagenesis, but the affected gene could not be isolated. Our mutant, like the previous ones, is in stkA. Its defect is cell-autonomous and not overcome by overexpressing cAMP-dependent protein kinase. stkA is strongly expressed in the prespore region of aggregates but not in the anterior prestalk zone. The mutant expresses normal levels of prespore-cell transcripts but fails to produce the spore transcript spiA. stkA encodes a predicted 99 kDa protein (STKA) with two putative C4 zinc fingers, one of which is a GATA-type finger, indicating that it may be a transcription factor. This conclusion is supported by localization of STKA in the nucleus.

原文English
頁(從 - 到)471-481
頁數11
期刊Cell
87
發行號3
DOIs
出版狀態Published - 1996 十一月 1

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

指紋 深入研究「Identification of the cell fate gene stalky in dictyostelium」主題。共同形成了獨特的指紋。

引用此