Imaging MS Analysis in Catharanthus roseus

Kotaro Yamamoto, Katsutoshi Takahashi, Sarah E. O’Connor, Tetsuro Mimura

研究成果: Chapter

摘要

To understand how the plant regulates metabolism, it is important to determine where metabolites localize in the tissues and cells. Single-cell level omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in Catharanthus roseus stem and leaf tissues.

原文English
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面33-43
頁數11
DOIs
出版狀態Published - 2022

出版系列

名字Methods in Molecular Biology
2505
ISSN(列印)1064-3745
ISSN(電子)1940-6029

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學

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