Immune modulation of recombinant OmpA against Brucella Abortus 544 infection in mice

Hannah Leah Tadeja Simborio, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Wongi Min, Hu Jang Lee, Jin Ju Lee, Hong Hee Chang, Suk Kim

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Brucellosis affects a wide range of host species, including humans and many livestock animals. Chronic infections of the disease make antibiotic treatment costly, and the current vaccine used in livestock has not been approved for human use. This study investigated the possible use of the Brucella abortus outer membrane protein A (OmpA) as a candidate subunit vaccine in an infected mouse model. The ompA gene was cloned and overexpressed, and the recombinant OmpA (rOmpA) protein fused to maltose binding protein (MBP) was purified in Escherichia coli. Immunogenicity was verified through western blotting, and mice were immunized and challenged to evaluate its protective effect. Mice treated with rOmpA exhibited induced humoral and host cell-mediated responses, with a significant increase in immunoglobulin G (IgG1 and IgG2a) and cytokine levels, especially TNF-α and IL-12, compared with the control groups treated with either MBP or PBS. In conclusion, rOmpA should be highly considered as a future subunit vaccine for brucellosis, and further studies regarding rOmpA and its protective ability are suggested.

原文English
頁(從 - 到)603-609
頁數7
期刊Journal of Microbiology and Biotechnology
26
發行號3
DOIs
出版狀態Published - 2015 12月 23

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 應用微生物與生物技術

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