Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates

Ai Hsiang Chou, Chia Chyi Liu, Jui Yuan Chang, Shu Pei Lien, Meng Shin Guo, Hau Pong Tasi, Kuang Nan Hsiao, Shih Jen Liu, Charles Sia, Suh Chin Wu, Min Shi Lee, Chia Hsin Hsiao, Jen Ren Wang, Yen Hung Chow, Pele Chong

研究成果: Article

30 引文 斯高帕斯(Scopus)

摘要

Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot, and mouth diseases (HFMDs), and EV71 is now recognized as an emerging neurotropic virus in Asia. Effective medications and/or prophylactic vaccines against HFMD are not available. The current results from mouse immunogenicity studies using in-house standardized RD cell virus neutralization assays indicate that (1) VP1 peptide (residues 211225) formulated with Freund's adjuvant (CFA/IFA) elicited low virus neutralizing antibody response (1/32 titer); (2) recombinant virus-like particles produced from baculovirus formulated with CFA/IFA could elicit good virus neutralization titer (1/160); (3) individual recombinant EV71 antigens (VP1, VP2, and VP3) formulated with CFA/IFA, only VP1 elicited antibody response with 1/128 virus neutralization titer; and (4) the formalin-inactivated EV71 formulated in alum elicited antibodies that cross-neutralized different EV71 genotypes (1/640), but failed to neutralize CVA16. In contrast, rabbits antisera could cross-neutralize strongly against different genotypes of EV71 but weakly against CVA16, with average titers 1/6400 and 1/32, respectively. The VP1 amino acid sequence dissimilarity between CVA16 and EV71 could partially explain why mouse antibodies failed to cross-neutralize CVA16. Therefore, the best formulation for producing cost-effective HFMD vaccine is a combination of formalin-inactivated EV71 and CAV16 virions.

原文English
文章編號831282
期刊Clinical and Developmental Immunology
2012
DOIs
出版狀態Published - 2012 十月 17

    指紋

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

引用此

Chou, A. H., Liu, C. C., Chang, J. Y., Lien, S. P., Guo, M. S., Tasi, H. P., Hsiao, K. N., Liu, S. J., Sia, C., Wu, S. C., Lee, M. S., Hsiao, C. H., Wang, J. R., Chow, Y. H., & Chong, P. (2012). Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates. Clinical and Developmental Immunology, 2012, [831282]. https://doi.org/10.1155/2012/831282