Impact of pH regulation on multicopper oxidase production and swarming motility in the bacterium Proteus hauseri ZMd44

Chang Su, Zhiyang Chen, I. Son Ng

研究成果: Article同行評審

摘要

Proteus hauseri ZMd44, a biodecolorizing bacterium, has been known to produce electricity and multicopper oxidase (Mco-laccase) under copper induction. However, optimization and regulation of production have not been explored. This study is the first attempt to evaluate several parameters on biomass and Mco-laccase production of P. hauseri ZMd44. Through orthogonal experiments with Taguchi's L9, it was found that P. hauseri ZMd44 was sensitive to pH value. The cells grew relatively quickly at pH 7, thus the biomass and Mco-laccase production reached 1.66 g/L and 1043.6 U/L, respectively. Higher pH values also influenced the swarming motility, which is an important characteristic of P. hauseri ZMd44 that affects urinary tract infection. The swarming circle and the diameter of the swarm, represented by the motility velocity, were found to be more controlled after 24 h of growth at pH 6. The swarming ability of P. hauseri was completely inhibited by the addition of 3 mM copper or zinc ions. Therefore, the Mco-laccase and swarming motility could be controlled by regulating pH and ion content.

原文English
頁(從 - 到)212-219
頁數8
期刊Biotechnology and Applied Biochemistry
65
發行號2
DOIs
出版狀態Published - 2018 三月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物工程
  • 分子醫學
  • 生物醫學工程
  • 應用微生物與生物技術
  • 藥物發現
  • 製程化學與技術

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