TY - JOUR
T1 - Impact of uremic environment on peritoneum
T2 - A proteomic view
AU - Wang, Hsien Yi
AU - Lin, Ching Yih
AU - Chien, Chih Chiang
AU - Kan, Wei Chih
AU - Tian, Yu Feng
AU - Liao, Pao Chi
AU - Wu, Hsin Yi
AU - Su, Shih Bin
N1 - Funding Information:
This research was supported by grants CMNCKU9909 and CMFHR-9806 from the Chi-Mei Medical Center . The authors thank the National Cheng-Kung University Proteomics Research Core Laboratory for assistance with two-dimensional gel electrophoresis and mass spectrometry analysis.
PY - 2012/4/3
Y1 - 2012/4/3
N2 - Peritoneal morphology and function are abnormal in uremia patients, but the contributing mechanisms are unclear. Here we attempted to characterize the protein targets that may be related to peritoneal change in patients with uremia and have not exposed to peritoneal dialysis fluid. Protein profiles of peritoneal fluids collected from patients with uremia and patients with normal renal function receiving laparoscopic cholecystectomy were displayed by two-dimensional gel electrophoresis (2-DE). Altered protein spots were excised and subjected to tryptic digestion followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Sixteen 2-DE protein spots were altered between two groups. Western blots confirmed that kininogen-1, apoptosis inhibitor 2, cat eye syndrome critical region protein 1, and apolipoprotein A-I had higher expression levels in the uremia samples. In contrast, synaptic vesicle 2-related protein, glial fibrillary acidic protein, and envelope glycoprotein (C2-V5 region) showed lower levels. The increased expression may result from a change in the permeability of the peritoneal membrane to middle-sized proteins or peritoneal inflammation with proteins sloughing off. All the identified proteins may provide a novel understanding of peritoneal changes caused by uremic toxins and may function as biomarkers or drug targets.
AB - Peritoneal morphology and function are abnormal in uremia patients, but the contributing mechanisms are unclear. Here we attempted to characterize the protein targets that may be related to peritoneal change in patients with uremia and have not exposed to peritoneal dialysis fluid. Protein profiles of peritoneal fluids collected from patients with uremia and patients with normal renal function receiving laparoscopic cholecystectomy were displayed by two-dimensional gel electrophoresis (2-DE). Altered protein spots were excised and subjected to tryptic digestion followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Sixteen 2-DE protein spots were altered between two groups. Western blots confirmed that kininogen-1, apoptosis inhibitor 2, cat eye syndrome critical region protein 1, and apolipoprotein A-I had higher expression levels in the uremia samples. In contrast, synaptic vesicle 2-related protein, glial fibrillary acidic protein, and envelope glycoprotein (C2-V5 region) showed lower levels. The increased expression may result from a change in the permeability of the peritoneal membrane to middle-sized proteins or peritoneal inflammation with proteins sloughing off. All the identified proteins may provide a novel understanding of peritoneal changes caused by uremic toxins and may function as biomarkers or drug targets.
UR - http://www.scopus.com/inward/record.url?scp=84862826334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862826334&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.01.011
DO - 10.1016/j.jprot.2012.01.011
M3 - Article
C2 - 22266485
AN - SCOPUS:84862826334
VL - 75
SP - 2053
EP - 2063
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
SN - 1874-3919
IS - 7
ER -