Impaired responsiveness of platelets to epinephrine due to α2A adrenoreceptor deficiency in Male Chinese

Tsun Mei Lin, Jih Shyan Lin, Jen Yu Tseng, Shang-Yin Wu, Tsai-Yun Chen

研究成果: Article

4 引文 (Scopus)

摘要

Epinephrine is known as a weak, but important, agonist for platelet activation. It has been reported that the responsiveness of platelets to epinephrine was markedly impaired in 6% of Caucasians and in 16% of Japanese. The purpose of this study was to screen and characterize this abnormality in healthy Taiwanese Chinese volunteers. We used aggregometry, flow cytometry and platelet function analyzer (PFA)-100 system to assess in 50 healthy male volunteers the responsiveness of platelets to epinephrine stimulation. Using α2A adrenoceptor antagonist BRL44408 maleate competition and a [3H]yohimbin binding assay, we evaluated α2A adrenoceptors on platelets. The aggregation of platelets after stimulation with 10 μM of epinephrine indicated two distinct groups of study participants: 24 (48.0%) good-and 26 (52.0%) impaired-responders to epinephrine. Flow cytometric analysis of platelets after stimulated with 1 μM epinephrine showed that glycoprotein (GP) IIb/IIIa and P-selectin expression of epinephrine good-and impaired-responders were 27.1 ± 11.0% vs. 9.9 ± 5.4% (p = 0.003) and 12.2 ± 6.2% vs. 3.6 ± 3.5% (p < 0.001), respectively. The PFA-100 system showed that epinephrine-impaired-responders had a longer collagen-epinephrine induced closure time. Good-responder platelets incubated with BRL44408 maleate had an impaired response to epinephrine stimulation. [3H]yohimbine binding studies showed fewer α2A adrenoreceptors on the platelets of epinephrine-impaired-responders than on those of good-responders. The prevalence of impaired responsiveness to epinephrine was high and probably due to α2A adrenoreceptor deficiency in male Taiwanese Chinese.

原文English
頁(從 - 到)149-154
頁數6
期刊Platelets
27
發行號2
DOIs
出版狀態Published - 2016 二月 17

指紋

Epinephrine
Blood Platelets
Adrenergic Receptors
Platelet Glycoprotein GPIIb-IIIa Complex
P-Selectin
Yohimbine
Platelet Activation
Platelet Aggregation
Volunteers
Healthy Volunteers
Flow Cytometry
Collagen

All Science Journal Classification (ASJC) codes

  • Hematology

引用此文

@article{84a50f790ac048e6b3f89ddf3ae0d369,
title = "Impaired responsiveness of platelets to epinephrine due to α2A adrenoreceptor deficiency in Male Chinese",
abstract = "Epinephrine is known as a weak, but important, agonist for platelet activation. It has been reported that the responsiveness of platelets to epinephrine was markedly impaired in 6{\%} of Caucasians and in 16{\%} of Japanese. The purpose of this study was to screen and characterize this abnormality in healthy Taiwanese Chinese volunteers. We used aggregometry, flow cytometry and platelet function analyzer (PFA)-100 system to assess in 50 healthy male volunteers the responsiveness of platelets to epinephrine stimulation. Using α2A adrenoceptor antagonist BRL44408 maleate competition and a [3H]yohimbin binding assay, we evaluated α2A adrenoceptors on platelets. The aggregation of platelets after stimulation with 10 μM of epinephrine indicated two distinct groups of study participants: 24 (48.0{\%}) good-and 26 (52.0{\%}) impaired-responders to epinephrine. Flow cytometric analysis of platelets after stimulated with 1 μM epinephrine showed that glycoprotein (GP) IIb/IIIa and P-selectin expression of epinephrine good-and impaired-responders were 27.1 ± 11.0{\%} vs. 9.9 ± 5.4{\%} (p = 0.003) and 12.2 ± 6.2{\%} vs. 3.6 ± 3.5{\%} (p < 0.001), respectively. The PFA-100 system showed that epinephrine-impaired-responders had a longer collagen-epinephrine induced closure time. Good-responder platelets incubated with BRL44408 maleate had an impaired response to epinephrine stimulation. [3H]yohimbine binding studies showed fewer α2A adrenoreceptors on the platelets of epinephrine-impaired-responders than on those of good-responders. The prevalence of impaired responsiveness to epinephrine was high and probably due to α2A adrenoreceptor deficiency in male Taiwanese Chinese.",
author = "Lin, {Tsun Mei} and Lin, {Jih Shyan} and Tseng, {Jen Yu} and Shang-Yin Wu and Tsai-Yun Chen",
year = "2016",
month = "2",
day = "17",
doi = "10.3109/09537104.2015.1049137",
language = "English",
volume = "27",
pages = "149--154",
journal = "Platelets",
issn = "0953-7104",
publisher = "Informa Healthcare",
number = "2",

}

Impaired responsiveness of platelets to epinephrine due to α2A adrenoreceptor deficiency in Male Chinese. / Lin, Tsun Mei; Lin, Jih Shyan; Tseng, Jen Yu; Wu, Shang-Yin; Chen, Tsai-Yun.

於: Platelets, 卷 27, 編號 2, 17.02.2016, p. 149-154.

研究成果: Article

TY - JOUR

T1 - Impaired responsiveness of platelets to epinephrine due to α2A adrenoreceptor deficiency in Male Chinese

AU - Lin, Tsun Mei

AU - Lin, Jih Shyan

AU - Tseng, Jen Yu

AU - Wu, Shang-Yin

AU - Chen, Tsai-Yun

PY - 2016/2/17

Y1 - 2016/2/17

N2 - Epinephrine is known as a weak, but important, agonist for platelet activation. It has been reported that the responsiveness of platelets to epinephrine was markedly impaired in 6% of Caucasians and in 16% of Japanese. The purpose of this study was to screen and characterize this abnormality in healthy Taiwanese Chinese volunteers. We used aggregometry, flow cytometry and platelet function analyzer (PFA)-100 system to assess in 50 healthy male volunteers the responsiveness of platelets to epinephrine stimulation. Using α2A adrenoceptor antagonist BRL44408 maleate competition and a [3H]yohimbin binding assay, we evaluated α2A adrenoceptors on platelets. The aggregation of platelets after stimulation with 10 μM of epinephrine indicated two distinct groups of study participants: 24 (48.0%) good-and 26 (52.0%) impaired-responders to epinephrine. Flow cytometric analysis of platelets after stimulated with 1 μM epinephrine showed that glycoprotein (GP) IIb/IIIa and P-selectin expression of epinephrine good-and impaired-responders were 27.1 ± 11.0% vs. 9.9 ± 5.4% (p = 0.003) and 12.2 ± 6.2% vs. 3.6 ± 3.5% (p < 0.001), respectively. The PFA-100 system showed that epinephrine-impaired-responders had a longer collagen-epinephrine induced closure time. Good-responder platelets incubated with BRL44408 maleate had an impaired response to epinephrine stimulation. [3H]yohimbine binding studies showed fewer α2A adrenoreceptors on the platelets of epinephrine-impaired-responders than on those of good-responders. The prevalence of impaired responsiveness to epinephrine was high and probably due to α2A adrenoreceptor deficiency in male Taiwanese Chinese.

AB - Epinephrine is known as a weak, but important, agonist for platelet activation. It has been reported that the responsiveness of platelets to epinephrine was markedly impaired in 6% of Caucasians and in 16% of Japanese. The purpose of this study was to screen and characterize this abnormality in healthy Taiwanese Chinese volunteers. We used aggregometry, flow cytometry and platelet function analyzer (PFA)-100 system to assess in 50 healthy male volunteers the responsiveness of platelets to epinephrine stimulation. Using α2A adrenoceptor antagonist BRL44408 maleate competition and a [3H]yohimbin binding assay, we evaluated α2A adrenoceptors on platelets. The aggregation of platelets after stimulation with 10 μM of epinephrine indicated two distinct groups of study participants: 24 (48.0%) good-and 26 (52.0%) impaired-responders to epinephrine. Flow cytometric analysis of platelets after stimulated with 1 μM epinephrine showed that glycoprotein (GP) IIb/IIIa and P-selectin expression of epinephrine good-and impaired-responders were 27.1 ± 11.0% vs. 9.9 ± 5.4% (p = 0.003) and 12.2 ± 6.2% vs. 3.6 ± 3.5% (p < 0.001), respectively. The PFA-100 system showed that epinephrine-impaired-responders had a longer collagen-epinephrine induced closure time. Good-responder platelets incubated with BRL44408 maleate had an impaired response to epinephrine stimulation. [3H]yohimbine binding studies showed fewer α2A adrenoreceptors on the platelets of epinephrine-impaired-responders than on those of good-responders. The prevalence of impaired responsiveness to epinephrine was high and probably due to α2A adrenoreceptor deficiency in male Taiwanese Chinese.

UR - http://www.scopus.com/inward/record.url?scp=84959112593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959112593&partnerID=8YFLogxK

U2 - 10.3109/09537104.2015.1049137

DO - 10.3109/09537104.2015.1049137

M3 - Article

C2 - 26083800

AN - SCOPUS:84959112593

VL - 27

SP - 149

EP - 154

JO - Platelets

JF - Platelets

SN - 0953-7104

IS - 2

ER -