Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination

Chia Yuan Chang, Chun Yu Lin, Vladimir A. Hovhannisyan, Shean Jen Chen

研究成果: Conference contribution

摘要

A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 µm to 1.5 µm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanning-based TFMPM to 1.5 µm from optimal 3.0 µm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.

原文English
主出版物標題European Conference on Biomedical Optics, ECBO_2019
發行者OSA - The Optical Society
ISBN(電子)9781557528209
DOIs
出版狀態Published - 2019 一月 1
事件European Conference on Biomedical Optics, ECBO_2019 - Munich, Netherlands
持續時間: 2019 六月 232019 六月 25

出版系列

名字Optics InfoBase Conference Papers
Part F142-ECBO 2019

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO_2019
國家Netherlands
城市Munich
期間19-06-2319-06-25

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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  • 引用此

    Chang, C. Y., Lin, C. Y., Hovhannisyan, V. A., & Chen, S. J. (2019). Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. 於 European Conference on Biomedical Optics, ECBO_2019 (Optics InfoBase Conference Papers; 卷 Part F142-ECBO 2019). OSA - The Optical Society. https://doi.org/10.1117/12.2526908