Improvement of axial excitation confinement in temporal focusing-based multiphoton microscopy via spatially modulated illumination

Chia-Yuan Chang, Shean Jen Chen

研究成果: Conference contribution

摘要

Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement (AEC) of a few microns. Herein, a developed TFMPEM with a digital micromirror device (DMD), acting as the blazed grating for light spatial dispersion and simultaneous patterned illumination, has been extended to implement spatially modulated illumination at structured frequency and orientation. By implementing the spatially modulated illumination, the beam coverage at the back-focal aperture of the objective lens can be increased. As a result, the AEC can be condensed from 3.0 μm to 1.5 μm in full width at half maximum for a 2-fold enhancement. Furthermore, by using HiLo microscopy with two structured illuminations at the same spatial frequency but different orientation, biotissue images according to the structured illumination with condensed AEC is obviously superior in contrast and scattering suppression.

原文English
主出版物標題Emerging Digital Micromirror Device Based Systems and Applications IX
編輯Michael R. Douglass, Benjamin L. Lee
發行者SPIE
ISBN(電子)9781510606753
DOIs
出版狀態Published - 2017 一月 1
事件Emerging Digital Micromirror Device Based Systems and Applications IX - San Francisco, United States
持續時間: 2017 一月 302017 一月 31

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
10117
ISSN(列印)0277-786X
ISSN(電子)1996-756X

Conference

ConferenceEmerging Digital Micromirror Device Based Systems and Applications IX
國家United States
城市San Francisco
期間17-01-3017-01-31

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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