In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

Tien Chun Tsai, Chia Wei Liu, Yi Chen Wu, Neil Adrian P. Ondevilla, Masatoshi Osawa, Hsien Chang Chang

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

原文English
頁(從 - 到)300-305
頁數6
期刊Colloids and Surfaces B: Biointerfaces
175
DOIs
出版狀態Published - 2019 三月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 表面和介面
  • 物理與理論化學
  • 膠體和表面化學

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