In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats

Tsung Hsing Lee, Lin Cheng Yang, An Kuo Chou, Ping-Ching Wu, Chung Ren Lin, Cheng Haung Wang, Jing Tsang Chen, Chao Shun Tang

研究成果: Article

16 引文 (Scopus)

摘要

Opioids remain the most efficacious pharmacological agents for various clinical pain syndromes. Recently, various engineered cells capable of secreting opioidergic peptides have been applied to relieve pain in animal models. In vivo gene delivery by viruses encoding endogenous opioids has also been used with success. In this study, we attempted non-viral intrathecal in vivo gene delivery by electroporation to induce analgesia. Thirty Sprague-Dawley rats were used in this study, six in each of five groups. Rats were treated as follows: vehicle without electroporation (group A), vehicle with electroporation (group B), 100μg of pCMV-hPOMC plasmid without electroporation (group C), or 100μg of pCMV-hPOMC plasmid with electroporation (group D). Group E was treated with both pCMV-hPOMC plasmid and electroporation, and given naloxone (1mg/kg) 1h before the formalin test. The tail flick, paw withdrawal latency from radiant heat, and formalin test results for each groups were compared. Radioimmunoassay (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the levels of expression of β-endorphin in the spinal cord. β-Endorphin expression was localized by immunohistochemistry. A significant decrease in the number of flinches in phase 2 of the formalin test was observed in the group treated with both plasmid and electroporation (group D), whereas the other measures of pain did not differ between groups. RIA and RT-PCR both showed increased expression of β-endorphin in group D. The expression of β-endorphin was highest in laminae I and II of the dorsal horn of the spinal cord. We conclude that electroporation successfully delivered intrathecally administered pCMV-hPOMC into the dorsal horn cells of the spinal cord, and induced analgesia in phase 2 of the formalin test in rats.

原文English
頁(從 - 到)159-167
頁數9
期刊Pain
104
發行號1-2
DOIs
出版狀態Published - 2003 一月 1

指紋

Pro-Opiomelanocortin
Electroporation
Analgesia
Formaldehyde
Endorphins
Pain
Injections
Pain Measurement
Plasmids
Opioid Analgesics
Reverse Transcription
Radioimmunoassay
Spinal Cord
Substantia Gelatinosa
Posterior Horn Cells
Polymerase Chain Reaction
Naloxone
Genes
Sprague Dawley Rats
Tail

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

引用此文

Lee, Tsung Hsing ; Yang, Lin Cheng ; Chou, An Kuo ; Wu, Ping-Ching ; Lin, Chung Ren ; Wang, Cheng Haung ; Chen, Jing Tsang ; Tang, Chao Shun. / In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats. 於: Pain. 2003 ; 卷 104, 編號 1-2. 頁 159-167.
@article{dd8240353f2b4ad894ae609867277868,
title = "In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats",
abstract = "Opioids remain the most efficacious pharmacological agents for various clinical pain syndromes. Recently, various engineered cells capable of secreting opioidergic peptides have been applied to relieve pain in animal models. In vivo gene delivery by viruses encoding endogenous opioids has also been used with success. In this study, we attempted non-viral intrathecal in vivo gene delivery by electroporation to induce analgesia. Thirty Sprague-Dawley rats were used in this study, six in each of five groups. Rats were treated as follows: vehicle without electroporation (group A), vehicle with electroporation (group B), 100μg of pCMV-hPOMC plasmid without electroporation (group C), or 100μg of pCMV-hPOMC plasmid with electroporation (group D). Group E was treated with both pCMV-hPOMC plasmid and electroporation, and given naloxone (1mg/kg) 1h before the formalin test. The tail flick, paw withdrawal latency from radiant heat, and formalin test results for each groups were compared. Radioimmunoassay (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the levels of expression of β-endorphin in the spinal cord. β-Endorphin expression was localized by immunohistochemistry. A significant decrease in the number of flinches in phase 2 of the formalin test was observed in the group treated with both plasmid and electroporation (group D), whereas the other measures of pain did not differ between groups. RIA and RT-PCR both showed increased expression of β-endorphin in group D. The expression of β-endorphin was highest in laminae I and II of the dorsal horn of the spinal cord. We conclude that electroporation successfully delivered intrathecally administered pCMV-hPOMC into the dorsal horn cells of the spinal cord, and induced analgesia in phase 2 of the formalin test in rats.",
author = "Lee, {Tsung Hsing} and Yang, {Lin Cheng} and Chou, {An Kuo} and Ping-Ching Wu and Lin, {Chung Ren} and Wang, {Cheng Haung} and Chen, {Jing Tsang} and Tang, {Chao Shun}",
year = "2003",
month = "1",
day = "1",
doi = "10.1016/S0304-3959(02)00496-7",
language = "English",
volume = "104",
pages = "159--167",
journal = "Pain",
issn = "0304-3959",
publisher = "Elsevier",
number = "1-2",

}

Lee, TH, Yang, LC, Chou, AK, Wu, P-C, Lin, CR, Wang, CH, Chen, JT & Tang, CS 2003, 'In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats', Pain, 卷 104, 編號 1-2, 頁 159-167. https://doi.org/10.1016/S0304-3959(02)00496-7

In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats. / Lee, Tsung Hsing; Yang, Lin Cheng; Chou, An Kuo; Wu, Ping-Ching; Lin, Chung Ren; Wang, Cheng Haung; Chen, Jing Tsang; Tang, Chao Shun.

於: Pain, 卷 104, 編號 1-2, 01.01.2003, p. 159-167.

研究成果: Article

TY - JOUR

T1 - In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats

AU - Lee, Tsung Hsing

AU - Yang, Lin Cheng

AU - Chou, An Kuo

AU - Wu, Ping-Ching

AU - Lin, Chung Ren

AU - Wang, Cheng Haung

AU - Chen, Jing Tsang

AU - Tang, Chao Shun

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Opioids remain the most efficacious pharmacological agents for various clinical pain syndromes. Recently, various engineered cells capable of secreting opioidergic peptides have been applied to relieve pain in animal models. In vivo gene delivery by viruses encoding endogenous opioids has also been used with success. In this study, we attempted non-viral intrathecal in vivo gene delivery by electroporation to induce analgesia. Thirty Sprague-Dawley rats were used in this study, six in each of five groups. Rats were treated as follows: vehicle without electroporation (group A), vehicle with electroporation (group B), 100μg of pCMV-hPOMC plasmid without electroporation (group C), or 100μg of pCMV-hPOMC plasmid with electroporation (group D). Group E was treated with both pCMV-hPOMC plasmid and electroporation, and given naloxone (1mg/kg) 1h before the formalin test. The tail flick, paw withdrawal latency from radiant heat, and formalin test results for each groups were compared. Radioimmunoassay (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the levels of expression of β-endorphin in the spinal cord. β-Endorphin expression was localized by immunohistochemistry. A significant decrease in the number of flinches in phase 2 of the formalin test was observed in the group treated with both plasmid and electroporation (group D), whereas the other measures of pain did not differ between groups. RIA and RT-PCR both showed increased expression of β-endorphin in group D. The expression of β-endorphin was highest in laminae I and II of the dorsal horn of the spinal cord. We conclude that electroporation successfully delivered intrathecally administered pCMV-hPOMC into the dorsal horn cells of the spinal cord, and induced analgesia in phase 2 of the formalin test in rats.

AB - Opioids remain the most efficacious pharmacological agents for various clinical pain syndromes. Recently, various engineered cells capable of secreting opioidergic peptides have been applied to relieve pain in animal models. In vivo gene delivery by viruses encoding endogenous opioids has also been used with success. In this study, we attempted non-viral intrathecal in vivo gene delivery by electroporation to induce analgesia. Thirty Sprague-Dawley rats were used in this study, six in each of five groups. Rats were treated as follows: vehicle without electroporation (group A), vehicle with electroporation (group B), 100μg of pCMV-hPOMC plasmid without electroporation (group C), or 100μg of pCMV-hPOMC plasmid with electroporation (group D). Group E was treated with both pCMV-hPOMC plasmid and electroporation, and given naloxone (1mg/kg) 1h before the formalin test. The tail flick, paw withdrawal latency from radiant heat, and formalin test results for each groups were compared. Radioimmunoassay (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the levels of expression of β-endorphin in the spinal cord. β-Endorphin expression was localized by immunohistochemistry. A significant decrease in the number of flinches in phase 2 of the formalin test was observed in the group treated with both plasmid and electroporation (group D), whereas the other measures of pain did not differ between groups. RIA and RT-PCR both showed increased expression of β-endorphin in group D. The expression of β-endorphin was highest in laminae I and II of the dorsal horn of the spinal cord. We conclude that electroporation successfully delivered intrathecally administered pCMV-hPOMC into the dorsal horn cells of the spinal cord, and induced analgesia in phase 2 of the formalin test in rats.

UR - http://www.scopus.com/inward/record.url?scp=0037823499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037823499&partnerID=8YFLogxK

U2 - 10.1016/S0304-3959(02)00496-7

DO - 10.1016/S0304-3959(02)00496-7

M3 - Article

C2 - 12855325

AN - SCOPUS:0037823499

VL - 104

SP - 159

EP - 167

JO - Pain

JF - Pain

SN - 0304-3959

IS - 1-2

ER -