摘要
Herpes simplex virus 1 (HSV-1) establishes latency in neurons of the brains and sensory ganglia of humans and experimentally infected mice. The latent virus can reactivate to cause recurrent infection. Both primary and recurrent infections can induce diseases, such as encephalitis. In humans, the majority of encephalitis cases occur as a recurrent infection. However, in the past, numerous mouse studies documented that viral reactivation occurs efficiently in the ganglion, but extremely rarely in the brain, when assessed ex vivo by cultivating minced tissue explants. Here, we compare the brains and the trigeminal ganglia of mice latently infected with HSV-1 (strain 294.1 or McKrae) for levels of viral genomes and in vivo reactivation. The numbers of copies of 294.1 and McKrae genomes in the brain stem were significantly greater than those in the trigeminal ganglion. Most importantly, 294.1 and McKrae reactivation was detected in the brain stems earlier than in the trigeminal ganglia of mice treated with hyperthermia to reactivate latent virus in vivo. In addition, the brain stem yielded reactivated virus at a high frequency compared with the trigeminal ganglion, especially in mice latently infected with 294.1 after hyperthermia treatment. These results provide evidence that recurrent brain infection can be induced by the reactivation of latent virus in the brain in situ.
| 原文 | English |
|---|---|
| 頁（從 - 到） | 11264-11270 |
| 頁數 | 7 |
| 期刊 | Journal of Virology |
| 卷 | 88 |
| 發行號 | 19 |
| DOIs | |
| 出版狀態 | Published - 2014 一月 1 |
指紋
All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Insect Science
- Virology
引用此文
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In vivo reactivation of latent Herpes simplex virus 1 in mice can occur in the brain before occurring in the trigeminal ganglion. / Yao, Hui Wen; Ling, Pin; Tung, Yuk-Ying; Hsu, Sheng-Min; Chen, Shun-hua.
於: Journal of Virology, 卷 88, 編號 19, 01.01.2014, p. 11264-11270.研究成果: Article
TY - JOUR
T1 - In vivo reactivation of latent Herpes simplex virus 1 in mice can occur in the brain before occurring in the trigeminal ganglion
AU - Yao, Hui Wen
AU - Ling, Pin
AU - Tung, Yuk-Ying
AU - Hsu, Sheng-Min
AU - Chen, Shun-hua
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Herpes simplex virus 1 (HSV-1) establishes latency in neurons of the brains and sensory ganglia of humans and experimentally infected mice. The latent virus can reactivate to cause recurrent infection. Both primary and recurrent infections can induce diseases, such as encephalitis. In humans, the majority of encephalitis cases occur as a recurrent infection. However, in the past, numerous mouse studies documented that viral reactivation occurs efficiently in the ganglion, but extremely rarely in the brain, when assessed ex vivo by cultivating minced tissue explants. Here, we compare the brains and the trigeminal ganglia of mice latently infected with HSV-1 (strain 294.1 or McKrae) for levels of viral genomes and in vivo reactivation. The numbers of copies of 294.1 and McKrae genomes in the brain stem were significantly greater than those in the trigeminal ganglion. Most importantly, 294.1 and McKrae reactivation was detected in the brain stems earlier than in the trigeminal ganglia of mice treated with hyperthermia to reactivate latent virus in vivo. In addition, the brain stem yielded reactivated virus at a high frequency compared with the trigeminal ganglion, especially in mice latently infected with 294.1 after hyperthermia treatment. These results provide evidence that recurrent brain infection can be induced by the reactivation of latent virus in the brain in situ.
AB - Herpes simplex virus 1 (HSV-1) establishes latency in neurons of the brains and sensory ganglia of humans and experimentally infected mice. The latent virus can reactivate to cause recurrent infection. Both primary and recurrent infections can induce diseases, such as encephalitis. In humans, the majority of encephalitis cases occur as a recurrent infection. However, in the past, numerous mouse studies documented that viral reactivation occurs efficiently in the ganglion, but extremely rarely in the brain, when assessed ex vivo by cultivating minced tissue explants. Here, we compare the brains and the trigeminal ganglia of mice latently infected with HSV-1 (strain 294.1 or McKrae) for levels of viral genomes and in vivo reactivation. The numbers of copies of 294.1 and McKrae genomes in the brain stem were significantly greater than those in the trigeminal ganglion. Most importantly, 294.1 and McKrae reactivation was detected in the brain stems earlier than in the trigeminal ganglia of mice treated with hyperthermia to reactivate latent virus in vivo. In addition, the brain stem yielded reactivated virus at a high frequency compared with the trigeminal ganglion, especially in mice latently infected with 294.1 after hyperthermia treatment. These results provide evidence that recurrent brain infection can be induced by the reactivation of latent virus in the brain in situ.
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UR - http://www.scopus.com/inward/citedby.url?scp=84906990873&partnerID=8YFLogxK
U2 - 10.1128/JVI.01616-14
DO - 10.1128/JVI.01616-14
M3 - Article
C2 - 25031345
AN - SCOPUS:84906990873
VL - 88
SP - 11264
EP - 11270
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 19
ER -