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In vivo RNA transcript-releasing plasmid possessing a universal pseudo-terminator by means of artificial ribozymes.

  • M. Ohme-Takagi
  • , H. Shinshi
  • , M. Oda
  • , T. Uchimaru
  • , S. Nishikawa
  • , K. Taira

研究成果: Article同行評審

1   連結會在新分頁中開啟 引文 斯高帕斯(Scopus)

摘要

RNA transcript-releasing plasmid has been constructed by means of artificial hammerhead ribozymes. In this specific construct of pGENE8459v3 the ribozyme targeted for SFL1 gene (a yeast suppressor gene for flocculation) was fused between two other ribozymes called 5'-processing and 3'-processing ribozymes. Since the "Ribozyme for SFL1" portion (cassette) can be replaced by other RNA sequences, it is now possible to produce any RNAs with defined 5'- and 3'- ends.

原文English
頁(從 - 到)49-50
頁數2
期刊Nucleic acids symposium series
發行號22
出版狀態Published - 1990

All Science Journal Classification (ASJC) codes

  • 一般醫學

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