@article{bb8a407c4ba84f3fae5f798fa783632b,
title = "In Vivo Visualization of Brain Vasculature in Alzheimer's Disease Mice by High-Frequency Micro-Doppler Imaging",
abstract = "Cerebrovascular disorders are associated with Alzheimer's disease (AD). Functional analysis of the cerebral vasculature requires an in vivo approach to visualize the blood flow in small animal brains. This paper proposes a high-frequency micro-Doppler imaging (HFμDI) technology for mapping mouse cerebral vasculature. Methods: HFμDI used a 40-MHz transducer with an ultrafast ultrasound imaging technology that enabled in vivo visualization of the mouse brain up to 3 mm in depth; furthermore, a minimal vessel diameter of 48 μm could be determined. Results: Animal experiments determined that the cortical and hippocampal vessel density in young wild-type (WT) mice was similar to that in middle-aged WT mice. However, compared with the vessel density in middle-aged WT mice, that in middle-aged mice with AD was significantly lower, particularly in the hippocampus. Discussion: In vivo observation of cerebral vasculature demonstrated the effectiveness of HFμDI for the preclinical study of AD, and a potential way for human diagnosis was provided.",
author = "Li, {Hsin Che} and Chen, {Pei Yu} and Cheng, {Hsiang Fan} and Kuo, {Yu Min} and Huang, {Chih Chung}",
note = "Funding Information: This work was supported in part by the National Health Research Institutes in Taiwan under Grant NHRI-EX107-10712EI Funding Information: Manuscript received October 29, 2018; revised January 21, 2019 and February 17, 2019; accepted March 9, 2019. Date of publication March 13, 2019; date of current version November 20, 2019. This work was supported in part by the National Health Research Institutes in Taiwan under Grant NHRI-EX107-10712EI, in part by the Ministry of Science and Technology of Taiwan under Grant MOST 107-2221-E-006-024-MY3, and in part by the Medical Device Innovation Center, National Cheng Kung University, from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan. (Corresponding author: Chih-Chung Huang.) H.-C. Li and P.-Y. Chen are with Department of Biomedical Engineering, College of Engineering, National Cheng Kung University. Publisher Copyright: {\textcopyright} 1964-2012 IEEE.",
year = "2019",
month = dec,
doi = "10.1109/TBME.2019.2904702",
language = "English",
volume = "66",
pages = "3393--3401",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
publisher = "IEEE Computer Society",
number = "12",
}