Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production

Ai Yun Lee, Cheng Yen Kao, Yao Kuan Wang, Ssu Yuan Lin, Tze Ying Lai, Bor-Shyang Sheu, Chien Jung Lo, Jiunn Jong Wu

研究成果: Article

3 引文 (Scopus)

摘要

Background: Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Materials and Methods: Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. Results: The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. Conclusions: These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching.

原文English
文章編號e12388
期刊Helicobacter
22
發行號4
DOIs
出版狀態Published - 2017 八月 1

指紋

Pylorus
Helicobacter pylori
Agar
Phase-Contrast Microscopy
Transmission Electron Microscopy
Culture Media
Iron
Observation
Bacteria
N-octanoylhomoserine lactone

All Science Journal Classification (ASJC) codes

  • Gastroenterology
  • Infectious Diseases

引用此文

Lee, Ai Yun ; Kao, Cheng Yen ; Wang, Yao Kuan ; Lin, Ssu Yuan ; Lai, Tze Ying ; Sheu, Bor-Shyang ; Lo, Chien Jung ; Wu, Jiunn Jong. / Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production. 於: Helicobacter. 2017 ; 卷 22, 編號 4.
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title = "Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production",
abstract = "Background: Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Materials and Methods: Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. Results: The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. Conclusions: These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching.",
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Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production. / Lee, Ai Yun; Kao, Cheng Yen; Wang, Yao Kuan; Lin, Ssu Yuan; Lai, Tze Ying; Sheu, Bor-Shyang; Lo, Chien Jung; Wu, Jiunn Jong.

於: Helicobacter, 卷 22, 編號 4, e12388, 01.08.2017.

研究成果: Article

TY - JOUR

T1 - Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production

AU - Lee, Ai Yun

AU - Kao, Cheng Yen

AU - Wang, Yao Kuan

AU - Lin, Ssu Yuan

AU - Lai, Tze Ying

AU - Sheu, Bor-Shyang

AU - Lo, Chien Jung

AU - Wu, Jiunn Jong

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Background: Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Materials and Methods: Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. Results: The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. Conclusions: These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching.

AB - Background: Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Materials and Methods: Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. Results: The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. Conclusions: These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching.

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