TY - JOUR
T1 - Induced pluripotent stem cell (iPSC) line MLi005-A derived from a patient with dominant dystrophic epidermolysis bullosa (DDEB)
AU - Ropret, Sandra
AU - Khurana, Preeti
AU - Fabčič, Tara
AU - Cvetkovska, Janina
AU - Trobec, Ana
AU - Jokhadar, Špela Zemljič
AU - Ilic, Dusko
AU - McGrath, John A.
AU - Guttmann-Gruber, Christina
AU - Liovic, Mirjana
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/3
Y1 - 2024/3
N2 - We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types.
AB - We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types.
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U2 - 10.1016/j.scr.2024.103306
DO - 10.1016/j.scr.2024.103306
M3 - Article
C2 - 38271763
AN - SCOPUS:85184837365
SN - 1873-5061
VL - 75
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 103306
ER -