Induction of 12-lipoxygenase expression by transforming growth factor-α in human epidermoid carcinoma A431 cells

Lei Chin Chen, Ben Kuen Chen, Yi Wen Liu, Wen Chang Chang

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

Transforming growth factor-α (TGF-α) increased the expression of 12-lipoxygenase activity in a time-dependent manner in human epidermoid carcinoma A431 cells. The increase of 12-lipoxygenase activity was accompanied by an increase in 12-lipoxygenase mRNA. The effect of TGF-α on the promoter activation of 12-lipoxygenase gene was analyzed by using the luciferase fusion vectors. A dose-dependent effect of TGF-α on the reporter activity was observed, which paralleled with its effect on enzyme activity. Transient transfection with a series of 5'-deleted constructs showed that the 5'-flanking region spanning from -224 to -100 bp from translation starting site played an important role for TGF-α response. Site-directed mutagenesis and gel mobility shift assay indicated that two Sp1 binding sequences residing at -158 to -150 bp and -123 to -114 bp were responsible for the TGF-α in activation of human 12-lipoxygenase gene transcription. Expression of Sp1, but not Sp3, stimulated the promoter activity of 12-lipoxygenase in SL2 cells, indicating that the binding of Sp1 with Sp1 binding sequences played a significant role in the regulation of 12-lipoxygenase gene. Copyright (C) 1999 Federation of European Biochemical Societies.

原文English
頁(從 - 到)105-110
頁數6
期刊FEBS Letters
455
發行號1-2
DOIs
出版狀態Published - 1999 7月 16

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學

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