Induction of apoptosis by 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3- propanedione through reactive oxygen species production, GADD153 expression, and caspases activation in human epidermoid carcinoma cells

Min Hsiung Pan, Yi Hong Sin, Ching Shu Lai, Ying-Jan Wang, Jen Kun Lin, Mingfu Wang, Chi Tang Ho

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

This study examined the growth inhibitory effects of the structurally related β-diketones compounds in human cancer cells. Here, we report that 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione (HMDB) induces growth inhibition of human cancer cells and induction of apoptosis in A431 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of HMDB-induced apoptosis, preceding cytochrome c release, caspase activation, and DNA fragmentation. The changes occurred after single breaks in DNA were detected, suggesting that HMDB induced irreparable DNA damage, which in turn triggered the process of apoptosis. Up-regulation of Bad and p21; down-regulation of Bcl-2, Bcl-X L , Bid, p53, and fatty acid synthase; and cleavage of Bax were found in HMDB-treated A431 cells. Glutathione and N-acetylcysteine (NAC) suppress HMDB-induced apoptosis. HMDB markedly enhanced growth arrest DNA damage inducible gene 153 (GADD153) mRNA and protein in a time- and concentration-dependent manner. NAC prevented up-regulation of GADD153 mRNA expression caused by HMDB. These findings suggest that HMDB creates an oxidative cellular environment that induces DNA damage and GADD153 gene activation, which in turn helps trigger apoptosis in A431 cells.

原文English
頁(從 - 到)9039-9049
頁數11
期刊Journal of Agricultural and Food Chemistry
53
發行號23
DOIs
出版狀態Published - 2005 十一月 16

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 農業與生物科學 (全部)

指紋

深入研究「Induction of apoptosis by 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3- propanedione through reactive oxygen species production, GADD153 expression, and caspases activation in human epidermoid carcinoma cells」主題。共同形成了獨特的指紋。

引用此